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Dahai GAO, Qingchun ZHANG, Zhongmin SUN. Development of chloroplast marker for identification of Ulva species[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2364-2371

Development of chloroplast marker for identification of Ulva species

Dahai GAO1,2, Qingchun ZHANG4, Zhongmin SUN3
1 Key Laboratory of Exploration and Utilization of Aquatic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;
2 Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China;
3 Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences(IOCAS), Qingdao 266071, China;
4 Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Large-scale Ulva-caused green tides has posed various environmental and ecological problems as well as economic consequences. It is important to identify them accurately and quickly. The current universal markers based on ITS, rbcL, and 5S spacer sequences were defective to distinct closely related species in the genus of Ulva. In this study, by investigating the intergenic regions of chloroplast (cp) genome, a novel marker of c15 (based on ycf3- rps7) were reliable for discrimination of Ulva species. Notably, the resolution of c15 was suitable for resolve the closely related species of U. linza and U. prolifera, which may provide tools for deciphering the blooming mechanisms of green tides.
Key words:    Ulva|linza-procera-prolifera (LPP) complex|molecular makers|ycf3-rps7 region   
Received: 2022-04-06   Revised:
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