Cite this paper:
Guanchao ZHENG, Haiyan WU, Mengmeng GUO, Jixing PENG, Yuxiu ZHAI, Zhijun TAN. First observation of domoic acid and its isomers in shellfish samples from Shandong Province, China[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2231-2241

First observation of domoic acid and its isomers in shellfish samples from Shandong Province, China

Guanchao ZHENG1, Haiyan WU1, Mengmeng GUO1, Jixing PENG1, Yuxiu ZHAI1,2,3, Zhijun TAN1,2,3
1 Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2 Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;
3 Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China
Atotal of 133 shellfish samples were collected in seven cities of Shandong Province, China, from May to October, 2019. The domoic acid (DA) concentrations were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and their distribution characteristics were investigated. DA concentration was detected high in over 1/3 (36.1%) of the samples of four kinds of shellfish in all three seasons in range from 0 to 102 μg/kg. The highest DA concentrations were 102, 101, 36.7, and 10.2 μg/kg in Crassostrea gigas, Chlamys farreri, Mactra veneriformis, and Mytilus edulis, respectively. Geographically, Yantai (22.0 μg/kg) and Weihai (16.9 μg/kg) showed relatively high concentrations of DA, whereas Rizhao and Dongying presented only 0.85- and 1.76-μg/kg DA, respectively. DA concentrations in the shellfish samples were strongly related to seasonal changes, being significantly higher in autumn and summer than that in spring. The DA risk exposure assessments indicate that dietary seafood consumption did not pose a health threat to general human population. In addition, three isomers (isoA, isoD, isoE) and 5'-epimer DA were detected in 3.00%–15.80% of the shellfish samples. This study is the first to observe DA and its isomers in shellfish samples of Shandong Province. The results demonstrate that DA contamination is very common and should be continuously monitored.
Key words:    domoic acid|shellfish|liquid chromatography-tandem mass spectrometry (LC-MS/MS)|Shandong Province   
Received: 2022-03-18   Revised:
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