Cite this paper:
Huixia GENG, Hongxiao SUN, Chao LIU, Fanzhou KONG, Qingchun ZHANG, Tian YAN, Rencheng YU. Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2218-2230

Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020

Huixia GENG1,4, Hongxiao SUN1,3, Chao LIU1,3, Fanzhou KONG1,2,4, Qingchun ZHANG1,2,4, Tian YAN1,2,4, Rencheng YU1,2,3,4
1 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China;
4 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Lipophilic marine toxins (LMTs) produced by some microalgae in the sea could accumulate in shellfish and pose potential threats to the health of seafood consumers. Phytoplankton and shellfish samples were collected from coastal waters of Weihai in Shandong Peninsula, China in autumn, 2020, and screened for lipophilic marine toxins and their potential producers using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and high throughput sequencing of partial DNA (V4 region of the 18S rRNA gene) extracted from phytoplankton. Pectenotoxin-2 (PTX2), trace amounts of azaspiracid (AZA1 or AZA40), and 13-desmethyl spirolide C (13-DesMe-C) were detected in phytoplankton samples, while PTX2 and gymnodimine (GYM) were detected in shellfish samples. The toxin content in shellfish samples was much lower than the regulatory limit or values reported previously. Results suggest that lipophilic marine toxins should have low risk in coastal waters of Weihai in autumn. Based on the data of high throughput sequencing, the OTUs were assigned to 5 identified species of Alexandrium, including A. ostenfeldii capable of producing 13-DesMe-C and GYM. Two OTUs were found closely related to the toxic species in genus Dinophysis, but it is impossible to assign them to any identified species due to the low resolving power of the V4 region for Dinophysis. The OTUs could not be assigned to any identified species in the genus Azadinium, suggesting the existence of unidentified species in this region.
Key words:    harmful algal blooms|phytoplankton|shellfish|lipophilic marine toxins (LMTs)|Yellow Sea|liquid chromatography-tandem mass spectrometry   
Received: 2022-08-17   Revised:
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