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Jian GAO, Yuelei DONG, Xiaoyu ZHOU, Lei CUI, Songhui LÜ. Biochemical composition of the brown tide causative species Aureococcus anophagefferens cultivated in different nitrogen sources[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2189-2201

Biochemical composition of the brown tide causative species Aureococcus anophagefferens cultivated in different nitrogen sources

Jian GAO1, Yuelei DONG1,3, Xiaoyu ZHOU1, Lei CUI1,3, Songhui LÜ1,2,3
1 Research Center of Harmful Algae and Marine Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;
2 Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai 519000, China;
3 Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China
A large-scale algal bloom, caused by Aureococcus anophagefferens, has plagued the coastal embayment of Qinhuangdao, China since 2009. The bay scallop agriculture industry in this area has been adversely affected. Researchers claimed that the poor nutritional value of brown tide cells might be responsible for the detrimental effects on bivalve mollusks. To verify whether brown tide cells are nutritionally inadequate food sources, the biochemical composition (total extractable lipids, amino acids, fatty acids, and monomeric carbohydrates) of the Chinese strain A. anophagefferens was determined during the late logarithmic growth phase when culturing in different nitrogen sources (nitrate, urea and nitrate-urea mixture). Cells cultured in nitrate contained 39.12% protein, 21.99% total extractable lipid, 10.25% total carbohydrates, and a relatively high amount of polyunsaturated fatty acid (PUFA) (51.98%, percentage of total fatty acids), including eicosapentaenoic acid (EPA) (4.81%) and docosahexaenoic acid (DHA) (14.56%). The gross biochemical composition and PUFA content in A. anophagefferens in nitrate cultivation are comparable with values found in the literature of frequently used species in bivalve feeding. Nine monomeric carbohydrates were significantly reduced when cultivated in urea and nitrate-urea mixture (P<0.05). The DHA, EPA, and PUFA contents significantly decreased when cultivated in urea (P<0.05). Although the nutritional value of A. anophagefferens dropped when cultured in urea, it is still comparable with certain favorably used algal species in bivalve feeding (i.e., Skeletonema costatum), indicating that A. anophagefferens is not a nutritionally inadequate food source.
Key words:    brown tide|Aureococcus anophagefferens|fatty acid|carbohydrate|nutritional value   
Received: 2021-10-18   Revised:
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