Cite this paper:
Jinlin JI, Zhenhui WANG, Wei ZHU, Qiang LI, Yinan WANG. Proteomic analysis provides insights into the function of Polian vesicles in the sea cucumber Apostichopus japonicus post-evisceration[J]. Journal of Oceanology and Limnology, 2022, 40(5): 2028-2041

Proteomic analysis provides insights into the function of Polian vesicles in the sea cucumber Apostichopus japonicus post-evisceration

Jinlin JI1,2, Zhenhui WANG1, Wei ZHU1, Qiang LI1, Yinan WANG1
1 College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China;
2 Dalian Ocean University, Dalian 116023, China
The Polian vesicle is the main accessory structure in the water vascular system of sea cucumbers. It can function to hold water vascular fluid under slight pressure and act as a hematopoiesis, excretory, and inflammatory response organ. Being the only organ to remain after evisceration, the Polian vesicle may function in the survival and regeneration of sea cucumber. We performed Tandem Mass Tag (TMT)-based proteomics to identify how proteins in the Polian vesicle of Apostichopus japonicus respond to evisceration. Among the 8 453 proteins identified from vesicle samples before evisceration (PV0h) and at 6-h post-evisceration (PV6h) and 3-d post-evisceration (PV3d), we detected 222 differentially abundant proteins (DAPs). Most of the annotated DAPs were associated with cell growth and proliferation, immune response and wound healing, substance transport and metabolism, cytoskeleton/cilia/flagella, extracellular matrix, energy production and conversion, protein synthesis and modification, and signal recognition and transduction. Compared with PV0h, fewer DAPs were identified at PV6h, and more DAPs were found at PV3d, and these DAPs were widely distributed among multiple biological processes. Our results indicate that a wide range of biological processes was induced in Polian vesicles in response to evisceration. In particular, Polian vesicles may play important roles in the restoration of coelomocytes, immune defense, and wound healing in sea cucumber. We propose that the Polian vesicle may be involved in visceral regeneration through nutrition and energy supply and by promoting dedifferentiation and migration. Together, these results provided new insights into the function of the Polian vesicle in A. japonicus post-evisceration.
Key words:    sea cucumber    Polian vesicle    evisceration    proteomics    differentially abundant protein   
Received: 2021-06-28   Revised: 2021-09-23
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