Cite this paper:
Peipei ZHAO, Qinghua WU, Xuekui XIA, Shiyi GUO, Sizhong SHEN, Yujue WANG, Aiyou HUANG. Metabolomic and proteomic responses of Phaeodactylum tricornutum to hypoxia[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1963-1973

Metabolomic and proteomic responses of Phaeodactylum tricornutum to hypoxia

Peipei ZHAO1, Qinghua WU1, Xuekui XIA1, Shiyi GUO2,3,4,5, Sizhong SHEN6, Yujue WANG2,3,4,5, Aiyou HUANG2,3,4,5,
1 Key Biosensor Laboratory of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China;
2 State Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, China;
3 Laboratory of Development and Utilization of Marine Microbial Resource, Hainan University, Haikou 570228, China;
4 Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, China;
5 College of Marine Sciences, Hainan University, Haikou 570228, China;
6 Department of Chemistry, University of Washington, Seattle WA 98195, USA
Diatoms are important contributors to global net primary productivity, and play a crucial role in the biogeochemical cycles of carbon, phosphorus, nitrogen, iron, and silicon. Currently in some regions in the ocean, there’s a trend that carbon content is high while oxygen concentration is low, and the underlying mechanisms of diatoms’ response to low oxygen environments are worth investigating. Phaeodactylum tricornutum is a model diatom whose genome has been sequenced; it provides a universal molecular toolbox and a stable transgenic expression system. Therefore, the study of the responses of P. tricornutum to low oxygen has not only fundamental research significance but also important ecological significance. In this study, growth rates were determined and proteomic analysis and metabolomic analysis were performed to examine P. tricornutum responses under different oxygen concentrations (2% oxygen concentration for hypoxic condition and 21% oxygen concentration for the normal condition (sterilized air)). Results show that the hypoxic environment inhibited the growth of P. tricornutum. In the hypoxic conditions, P. tricornutum could reset its metabolism pathways, including enhancement in lipid utilization, replenishment of tricarboxylic acid (TCA) cycle through the glyoxylic acid cycle, and down-regulation of photorespiration to reduce energy waste. Additionally, the stress resistance mechanism was activated to facilitate the adaptation to low oxygen conditions. This study helps to reveal the different metabolic changes to hypoxia of diatom from that of higher plants, which might be ascribed to their different habitats and needs further exploration in the future.
Key words:    Phaeodactylum tricornutum    hypoxia    metabolomics    proteomics   
Received: 2021-07-16   Revised: 2021-09-01
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