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Cite this paper: |
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Junqiong SHI, Shuhan HE, Lu ZHAO, Lulu JI, Songqi YANG, Zhongxing WU. Physiological and molecular responses of invasive cyanobacterium Raphidiopsis raciborskii to ambient phosphorus deficiency[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1792-1803 |
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Physiological and molecular responses of invasive cyanobacterium Raphidiopsis raciborskii to ambient phosphorus deficiency |
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| Junqiong SHI, Shuhan HE, Lu ZHAO, Lulu JI, Songqi YANG, Zhongxing WU |
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| Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing 400715, China |
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| Abstract: |
| Raphidiopsis raciborskii can cause harmful cyanobacterial blooms when concentrations of environmental phosphorus (P) are very low, thus the physiological and molecular mechanisms involved in the acclimation to P need to be characterized better. The growth, chlorophyll fluorescence, alkaline phosphatase, and expression of genes directly involved in P assimilation were compared in the R. raciborskii FACHB 1496 strain grown with and without inorganic P. The specific growth rate (μ), Chl a, and six fluorescence parameters (minimal fluorescence (F0), maximal fluorescence (Fm), maximal variable fluorescence (Fv), electron transport flux (further than QA) per RC (ET0/RC), quantum yield of the electron transport in PSⅡ (ØE0), and the probability that an electron from a trapped exciton is moved into the electron transport chain beyond QA - (ψ0)) markedly decreased in R. raciborskii in response to experimental P-deficiency. In contrast, the relative variable fluorescence at the J-step (VJ), trapped energy flux (leading to QA reduction) per RC (TR0/RC), and alkaline phosphatase activity significantly increased. In addition, gene expressions involved in the alkaline phosphatase (phoA1 and phoA2), high-affinity inorganic P transporter (pstS1), phosphonate transporter and metabolism (phnD and phnM), and nucleotidase (nucH) were significantly upregulated under P deficiency. However, physiological and molecular responses were resumed rapidly after P re-supplementation following P-deficient conditions. Our results highlight that R. raciborskii can perform coordinated and complex cellular and physiological responses to cope with P deficiency, reflecting R. raciborskii’s multi-faceted machinery to respond to environmental P fluctuations. |
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| Key words:
phosphorus deficiency
Raphidiopsis raciborskii
gene expression
chlorophyll fluorescence
alkaline phosphatase
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| Received: 2021-10-09 Revised: 2021-11-19 |
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