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Xuejiao LIANG, Chi ZHANG, Dongdong DU, Meirong GAO, Liying SUI. Application of biofloc technology in recirculation Artemia culture system[J]. Journal of Oceanology and Limnology, 2022, 40(4): 1669-1677

Application of biofloc technology in recirculation Artemia culture system

Xuejiao LIANG1, Chi ZHANG2, Dongdong DU1, Meirong GAO1, Liying SUI1
1 Key Laboratory of Marine Resource Chemistry and Food Technology(TUST), Ministry of Education;College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China;
2 Institute of Fisheries Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China
Biofloc technology (BFT) improves water quality, and productivity of the farmed species through converting ammonium nitrogen to microbial protein, stabilizing microbial community, and reducing the production cost. In this study, a small-scale biofloc development unit was designed in combination of recirculation system (RAS) for Artemia culture. Artemia growth, water quality, and microbial composition of bioflocs in RAS were studied in comparison with in-situ batch culture (Glu). Glucose was added in RAS and Glu at C/N ratio of 10. The cultures without glucose addition, but with 50% daily water renewal (WRe) and without water renewal (NWRe) were considered as the controls. Artemia were cultured at 25 ℃ and salinity 30 for 24 days and fed formulated feed. The results showed that compared to the controls, Glu significantly improved the Artemia biomass, increased the biofloc volume, and reduced the content of total ammonia nitrogen (TAN), nitrite nitrogen (NO2-N) and nitrate nitrogen (NO3-N) in water column (P<0.05). In addition, RAS had similar results with Glu. High throughput sequencing analysis on biofloc microbial composition demonstrated that glucose supplement shaped the microbial community structure, and increased proportion of potential probiotic bacteria and suppressed pathogenic bacteria growth. Furthermore, we analyzed the relationship between the microbial composition of biofloc and environmental factors. Canonical correspondence analysis (CCA) indicated that inorganic nitrogen in culture water had great impact on biofloc microbial composition in NWRe and WRe, whilst the dissolved organic carbon (DOC) modified the microbial community in Glu and RAS. This study shows the advantages of BFT in Artemia culture and provides practical information for applying BFT-RAS in indoor Artemia culture.
Key words:    biofloc technology|Artemia|water quality|microbial community structure   
Received: 2021-05-06   Revised:
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