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Xudan LU, Fangru NAN, Jia FENG, Junping LÜ, Qi LIU, Xudong LIU, Shulian XIE. Transcriptome analysis of Porphyridium purpureum under salinities of 0 and 68[J]. Journal of Oceanology and Limnology, 2022, 40(4): 1600-1614

Transcriptome analysis of Porphyridium purpureum under salinities of 0 and 68

Xudan LU, Fangru NAN, Jia FENG, Junping LÜ, Qi LIU, Xudong LIU, Shulian XIE
School of Life Science, Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan 030006, China
The most prominent biological characteristic of Porphyridium purpureum is their unique extracellular sulfated polysaccharides that plays an important protective role against salt stress. Adaptation to stress is associated with metabolic adjustments. However, the molecular mechanisms underlying such metabolic changes remain elusive. This study presents the first transcriptome profiling of P. purpureum. A total of 8 019 assembled transcripts were identified, annotated, and classified into different biological categories and pathways based on a BLAST analysis against various databases. The in-depth analysis revealed that many of the differentially expressed genes in P. purpureum under salinities of 68 and 0 involved polysaccharide metabolism. A comparison of the gene expression levels under different salinities revealed that the polysaccharide synthetic pathway was significantly upregulated under the 68 salinity condition. The increased expression of the FBP, pfkA, CS, pgm, USP, UGP2, galE, and MPI transcripts in the polysaccharide synthetic pathway and the increase in ATP2 and ATP6 transcripts in the energy metabolic pathway revealed the molecular mechanism of high-salt adaptation. This sequencing dataset and analysis could serve as a valuable resource to study the mechanisms involved in abiotic stress tolerance in Rhodophyta.
Key words:    transcriptome|salinity|polysaccharides|osmotic adjustment|enzyme activity   
Received: 2021-03-03   Revised:
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