Cite this paper:
Yu SHI, Xiaolan PAN, Meng XU, Huiru LIU, Hanzhi XU, Maoxian HE. The role of Smad6 in immunity of the pearl oyster Pinctada fucata martensii[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1135-1147

The role of Smad6 in immunity of the pearl oyster Pinctada fucata martensii

Yu SHI1,2, Xiaolan PAN1, Meng XU1, Huiru LIU1, Hanzhi XU1, Maoxian HE1,2
1 CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
2 Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou), Guangzhou 511458, China
Inhibitory Smads (I-Smads), which belong to the Smad family and inhibit bone morphogenic protein 2 (BMP2) signaling by a variety of mechanisms, can suppress innate immunity responses in vertebrates. However, there are no reports for the role of Smad6 in immunity in mollusks. In this study, we showed that Smad6 of the pearl oyster Pinctada fucata martensii was located in the Smad6 cluster of the phylogenetic tree; mRNA expression of Smad6 and Smad3 was up-regulated after lipopolysaccharide and polyinosinic: polycytidylic challenge; and transcript levels of Smad6 and Smad3 showed opposite patterns during wound healing. Under salinity stress, water inflow and outflow in the gills appear to be regulated by BMP2-Smads signals, and BMP2-Smads signaling may be closely related to the immune response. Our results indicate that Smad6 is involved in immunity, that it plays a positive role in the response to immune challenge and an inhibitory role during wound healing, and that Smad6 and Smad3 may work against each other.
Key words:    Smad6|BMP2-Smads signal pathway|expression|immunity|Pinctada fucata martensii   
Received: 2021-03-26   Revised:
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