Cite this paper:
Xudong LIU, Ran ZHANG, Jia FENG, Fabio RINDI, Shulian XIE. Neglectella glomeratasp. nov., a new species and implications for the systematics of the genus Neglectella (Oocystaceae, Trebouxiophyceae, Chlorophyta)[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2370-2379

Neglectella glomeratasp. nov., a new species and implications for the systematics of the genus Neglectella (Oocystaceae, Trebouxiophyceae, Chlorophyta)

Xudong LIU1, Ran ZHANG1, Jia FENG1, Fabio RINDI2, Shulian XIE1
1 School of Life Science, Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan 030006, China;
2 Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona 60131, Italy
The genus Neglectella Vodenicarov & Benderliev has a morphology similar to the well-known genus Oocystis, except for the numerous chloroplasts stacked in the surface layer of the cell. Neglectella subsequently experienced complex taxonomy changes with members being transferred in and out, and five species are now accepted. In this study, a new alga with morphology corresponding to this genus was collected in China and successfully cultured in laboratory. Morphological observations in light microscopy and transmission electronic microscopy (TEM) and phylogenetic analyses revealed that it is a new species, described here as Neglectella glomerata sp. nov. It differed from other Neglectella species due to a morphological difference in its unique mucilaginous colony with a large number of cells, multiple pyrenoids in each chloroplast, smaller cell size, and more elongated cell shape. Phylogenetic analyses placed this alga in the subfamily Eremosphaeroideae of the family Oocystaceae and showed its close relationship with Neglectella peisonis. Further morphological comparisons combined with phylogenetic results revealed three different morphological types in Neglectella. The new type represented by our new species may represent a transitional morphology in intermediate taxonomic position between the other two types. The diversity of morphological types in Neglectella suggests that further unknown species of this genus are likely to be discovered in the future. More collections and DNA sequence data will be needed in the future for this genus.
Key words:    colony organization|Eremosphaeroideae|molecular phylogeny|Neglectella|Oocystaceae|pyrenoid   
Received: 2020-09-20   Revised: 2020-11-14
PDF (2360 KB) Free
Print this page
Add to favorites
Email this article to others
Articles by Xudong LIU
Articles by Ran ZHANG
Articles by Jia FENG
Articles by Fabio RINDI
Articles by Shulian XIE
De Bary A. 1858. Untersuchungen über die familie der conjugaten (Zygnemeen und Desmidieen). In:Felix A ed.
Ein Beitrag Zur Physiologischen und Beschreibenden Botanik. Förstnersche Buchhandlung, Leipzig. p.1-91.
Faluaburu M S, Nakai R, Imura S, Naganuma T. 2019. Phylotypic characterization of mycobionts and photobionts of rock tripe lichen in East Antarctica. Microorganisms, 7(7):203,
Fott B. 1976. Oocystis und verwandte Gattungen aus der Unterfamilie der Oocystoideae; Namensänderungen, taxonomische Notizen und Bestimmungsschlüssel. Preslia, Praha, 48:193-206.
Guiry M D. 2020. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Accessed on 2020-07-13.
Hepperle D, Hegewald E, Krienitz L. 2000. Phylogenetic position of the oocystaceae (Chlorophyta). Journal of Phycology, 36(3):590-595,
Hoshaw R W, Rosowski J R. 1973. Methods for microscopic algae. In:Stein J R ed. Handbook of Phycological Methods. Cambridge University Press, New York. p.53-68.
Huelsenbeck J P, Ronquist F. 2001. MRBAYES:bayesian inference of phylogenetic trees. Bioinformatics, 17(8):754-755,
Iyengar M O P. 1932. Two little-known genera of green algae(Tetrasporidium and Ecballocystis). Annals of Botany, 46:191-192.
Komárek J, Fott B. 1983. Chlorophyceae (grünalgen), ordnung:chlorococcales. In:Huber-Petalozzi G, Heynig H, Mollenhauer D eds. Das Phytoplankton des Sübwassers.
Systematic und Biologie. E. Schweizerbart'sche Verlagsbuchhandlung, Stuttgart. p.1-1 044.
Korsikov A A. 1939. Contribution to the algal flora of the Gorky District Phytoplankton of the Oka River in August 1932. Zapiski Gorkovskogo Universiteta, 9:101-128.
Krienitz L, Bock C. 2011. Elongatocystis ecballocystiformis gen. et comb. nov., and some reflections on systematics of Oocystaceae (Trebouxiophyceae, Chlorophyta). Fottea, 11(2):271-278,
Larkin M A, Blackshields G, Brown N P, Chenna R, McGettigan P A, McWilliam H, Valentin F, Wallace I M, Wilm A, Lopez R, Thompson J D, Gibson T J, Higgins D G. 2007. Clustal W and clustal X version 2.0. Bioinformatics, 23(21):2947-2948,
Liu X D, Wang Q H, Zhu H, Liu B W, Rindi F, Liu G X, Xie S L, Hu Z Y. 2020. Reticulocystis yunnanense gen. et sp. nov., a new member of freshwater Oocystaceae algae(Trebouxiophyceae, Chlorophyta). European Journal of Phycology, 55(4):507-516,
Liu X D, Zhu H, Song H Y, Liu B W, Wang Q H, Liu G X, Hu Z Y. 2018. Quadricoccopsis gen. nov., a new genus of Quadricoccus-like algae in Oocystaceae from China(Trebouxiophyceae, Chlorophyta). Fottea, 18(2):189-199,
Liu X S, Zhu H, Liu B W, Liu G X, Hu Z Y. 2017. Classification of planctonema-like algae, including a new genus Planctonemopsis gen. nov., a new species Planctonema gelatinosum sp. nov. and a reinstated genus Psephonema(Trebouxiophyceae, Chlorophyta). Journal of Phycology, 53(4):869-879,
Liu Y, Liu Z H, Zhang J L, Hu Y Y, Sun H L. 2010. Experimental study on the utilization of DIC by Oocystis solitaria Wittr and its influence on the precipitation of calcium carbonate in karst and non-karst waters. Carbonates and Evaporites, 25(1):21-26,
Playfair G I. 1918. New and rare freshwater algae. Proceedings of the Linnean Society of New South Wales, 43:497-543.
Printz H. 1913. Eine systematische übersicht der gattung Oocystis nägeli. Nyt Magasin for Naturvidenskapene, 51:165-203.
Quader H, Robinson D G, van Kempen R. 1983. Cell wall development in Oocystis solitaria in the presence of polysaccharide binding dyes. Planta, 157(4):317-323,
Quader H. 1986. Cellulose microfibril orientation in Oocystis solitaria:proof that microtubules control the alignment of the terminal complexes. Journal of Cell Science, 83:223-234.
Reynolds E S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. The Journal of Cell Biology, 17(1):208-212,
Schagerl M. 1993. Lichtmikroskopische und ultrastrukturelle Untersuchungen an Neglectella peisonis spec. nov. - einer planktischen Oocystaceae aus dem Neusiedler See(Österreich). Nova Hedwigia, 56:61-74.
Spurr A R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research, 26(1-2):31-43,
Stamatakis A. 2014. RAxML version 8:a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30(9):1312-1313,
Stanier R Y, Kunisawa R, Mandel M, Cohen-Bazire G. 1971. Purification and properties of unicellular blue-green algae(Order Chroococcales). Bacteriological Reviews, 35(2):171-205,
Štenclová L, Fučíková K, Kaštovský J, Pažoutová M. 2017. Molecular and morphological delimitation and generic classification of the family Oocystaceae(Trebouxiophyceae, Chlorophyta). Journal of Phycology, 53(6):1263-1282,
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5:molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10):2731-2739,
Turmel M, Otis C, Lemieux C. 2009. The chloroplast genomes of the green algae Pedinomonas minor, Parachlorella kessleri, and Oocystis solitaria reveal a shared ancestry between the pedinomonadales and chlorellales. Molecular Biology and Evolution, 26(10):2317-2331,
Vodenicarov D, Benderliev K. 1971. Neglectella gen. nov.(Chlorococcales). Nova Hedwigia, 21:801-812.
Vodeničarov D. 1989. Die gattung Neglectella vodenic. et benderl. und zwei neue gattungen:neglectellopsis gen. nov. und Skujaster gen. nov. (Chlorophyta, Chlorococcales). Algological Studies, 57:409-424.
Xia S, Zhu H, Cheng Y Y, Liu G X, Zheng Y H. 2013. Phylogenetic position of Ecballocystis and Ecballocystopsis (Chlorophyta). Fottea, 13(1):65-75,
Copyright © Haiyang Xuebao