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Caixia WANG, Min WANG, Binbin CHEN, Wenli QIN, Lidong LIN, Chuanjun DAI, Hengguo YU, Renhui LI, Min ZHAO, Zengling MA. Harmful algal bloom-forming dinoflagellate Prorocentrum donghaiense inhibits the growth and photosynthesis of seaweed Sargassum fusiformis embryos[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2237-2251

Harmful algal bloom-forming dinoflagellate Prorocentrum donghaiense inhibits the growth and photosynthesis of seaweed Sargassum fusiformis embryos

Caixia WANG1,2, Min WANG1,2, Binbin CHEN1,2, Wenli QIN1,2, Lidong LIN1,3, Chuanjun DAI1,2, Hengguo YU1,2, Renhui LI1,2, Min ZHAO1,2, Zengling MA1,2
1 Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China;
2 National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Wenzhou University, Wenzhou 325035, China;
3 Dongtou Fisheries Science and Technology Research Institute, Wenzhou 325700, China
Harmful algal bloom (HAB) is an ecological disaster to local mariculture. At present, its impact on macrophytes has not been well studied. In this study, we cultivated sexually propagated embryos of Sargassum fusiformis— an edible seaweed—in Prorocentrum donghaiense suspensions at different cell densities (0, 0.50×105, 0.75×105, 1.00×105, and 1.50×105 cells/mL) for 10 days, during which growth and photosynthetic activities of the embryos were determined, and a monocultivation was set up for comparison. Results show that the relative growth rate and photosynthetic activities of the embryos co-cultivated with P. donghaiense were inhibited mostly and significantly in the cell densities of 0.75×105, 1.00×105, and 1.50×105 cells/mL, and the inhibitory effects increased in overall with increased cell densities. The maximum relative electron transport rates (rETRmax) and apparent photosynthetic efficiency (α) of co-cultivated embryos were all significantly lower than monocultivation ones on the 10th day. Furthermore, the photosynthetic activity detected by chlorophyll-a fluorescence transient (i.e., OJIP), the electron transport among electron transfer accepters of PSⅡ (photosystem Ⅱ) and that from PSⅡ to PSI (photosystem I) was restricted, which is probably responsible for the decreases of rETRmax and α in the co-cultivated embryos. In addition, parts of the photosynthetic reaction centers of PSⅡ in the co-cultivated embryos were inactivated. Therefore, P. donghaiense bloom could restrain the development and photosynthetic activities of S. fusiformis embryos, reduce the seedlings stock, and eventually hinder the development of S. fusiformis production industry.
Key words:    embryo|JIP-test|photosynthesis|Prorocentrum donghaiense|Sargassum fusiformis|harmful algal bloom   
Received: 2020-10-31   Revised: 2020-12-21
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