Cite this paper:
Haijian DU, Wenyan ZHANG, Wei LIN, Hongmiao PAN, Tian XIAO, Long-Fei WU. Genomic analysis of a pure culture of magnetotactic bacterium Terasakiella sp. SH-1[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2097-2106

Genomic analysis of a pure culture of magnetotactic bacterium Terasakiella sp. SH-1

Haijian DU1,2, Wenyan ZHANG2,3,4,7, Wei LIN5,6,7, Hongmiao PAN2,3,4,7, Tian XIAO2,3,4,7, Long-Fei WU7,8
1 College of Life Science, Shandong University, Qingdao 266237, China;
2 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
3 Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;
4 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
5 Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China;
6 Institutions of Earth Science, Chinese Academy of Sciences, Beijing 100029, China;
7 France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms, Chinese Academy of Sciences, Beijing 100029, China;
8 Aix-Marseille University, CNRS, LCB, Marseille F-13402, France
Magnetotactic bacteria (MTB) display magnetotaxis ability because of biomineralization of intracellular nanometer-sized, membrane-bound organelles termed magnetosomes. Despite having been discovered more than half a century, only a few representatives of MTB have been isolated and cultured in the laboratory. In this study, we report the genomic characterization of a novel marine magnetotactic spirillum strain SH-1 belonging to the genus Terasakiella that was recently isolated. A gene encoding haloalkane dehalogenase, which is involved in the degradation of chlorocyclohexane, chlorobenzene, chloroalkane, and chloroalkene, was identified. SH-1 genome contained cysCHI and soxBAZYX genes, thus potentially capable of assimilatory sulfate reduction to H2S and using thiosulfate as electron donors and oxidizing it to sulfate. Genome of SH-1 also contained genes encoding periplasmic dissimilatory nitrate reductases (napAB), assimilatory nitrate reductase (nasA) and assimilatory nitrite reductases (nasB), suggesting that it is capable of gaining energy by converting nitrate to ammonia. The pure culture of Terasakiella sp. SH-1 together with its genomic results offers new opportunities to examine biology, physiology, and biomineralization mechanisms of MTB.
Key words:    magnetotactic bacteria|magnetotaxis|pure culture|comparative genomic analysis   
Received: 2021-02-10   Revised: 2021-03-11
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