Cite this paper:
Sha WU, Qing WANG, Xu WANG, Ruixue GUO, Tongwei ZHANG, Yongxin PAN, Feng LI, Ying LI. MamZ protein plays an essential role in magnetosome maturation process of Magnetospirillum gryphiswaldense MSR-1[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2082-2096

MamZ protein plays an essential role in magnetosome maturation process of Magnetospirillum gryphiswaldense MSR-1

Sha WU1, Qing WANG2, Xu WANG2, Ruixue GUO1, Tongwei ZHANG3, Yongxin PAN3, Feng LI1, Ying LI2
1 Engineering Technology Research Center of Ecological Restoration and Comprehensive Utilization of Coal Mining Collapse Area, Anhui Province;Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China;
2 State Key Laboratories for Agro-biotechnology, China Agricultural University, Beijing 100193, China;
3 Paleomagnetism and Geochronology Laboratory, Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicelluar Organisms, Beijing 100029, China
Based on analysis of gene structure of mamXY operon in Magnetospirillum gryphiswaldense strain MSR-1, we constructed a mamZ deletion mutant strain (△mamZ) and four complemented strains with different mamZ fragment lengths. Various cell phenotypic and physiological parameters were evaluated and compared among the wild-type (WT), mutant, and complemented strains. Cell growth rates were not notably different; however, magnetic response (Cmag) and iron uptake ability were significantly lower in △mamZ. High-resolution transmission electron microscopy (HR-TEM) showed that magnetosomes in △mamZ were small and irregular, and rock magnetic measurements suggested that they contained immature particles. In comparison to WT of MSR-1, intracellular iron content of △mamZ and the complemented strains cultured with 20 μmol/L iron source was similar or slightly higher. The complemented strains were unable to synthesize mature or normal amounts of magnetosomes, apparently because of abnormal expression of the transmembrane domain of MamZ protein. Real-time reverse transcription polymerase chain reaction (RTqPCR) analysis showed that relative transcription levels of mamX and ftsZ-like genes in △mamZ were higher at 18 h than at 12 h, suggesting that MamXY proteins play cooperative functional roles in the magnetosome maturation process. Transcription level of mms6 was significantly upregulated in △mamZ (incubated at 12 h) and the complemented strains (incubated at 12 and 18 h), reflecting possible interaction between MamXY and Mms6 proteins during magnetosome biosynthesis. These findings, taken together, demonstrate the essential role of MamZ in the magnetosome maturation process in MSR-1.
Key words:    Magnetospirillum gryphiswaldense|mamZ|deletion|mamXY operon|magnetosome maturation   
Received: 2020-08-27   Revised: 2020-09-29
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