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Haitao CHEN, Jinhua LI, Long-Fei WU, Wei-Jia ZHANG. Morphological and phylogenetic diversity of magnetotactic bacteria and multicellular magnetotactic prokaryotes from a mangrove ecosystem in the Sanya River, South China[J]. Journal of Oceanology and Limnology, 2021, 39(6): 2015-2026

Morphological and phylogenetic diversity of magnetotactic bacteria and multicellular magnetotactic prokaryotes from a mangrove ecosystem in the Sanya River, South China

Haitao CHEN1,2,3, Jinhua LI2,4, Long-Fei WU2,5, Wei-Jia ZHANG2,3
1 Beijing Key Laboratory of Biological Electromagnetism, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100190, China;
2 France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms, Chinese Academy of Sciences, Beijing 100029, China;
3 Deep-Sea Microbial Cell Biology, Department of Deep Sea Sciences, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China;
4 Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China;
5 LCB, Aix Marseille University, CNRS, Marseille 13402, France
Magnetotactic bacteria (MTB) are morphologically and phylogenetically diverse prokaryotes commonly able to produce magnetic nanocrystals within intracellular membrane-bound organelles (i.e., magnetosomes) and to swim along geomagnetic field lines. We studied the diversity of MTB in the samples collected from a mangrove area in the Sanya River, Hainan, South China, using microscopic and microbial phylogenetic methods. Results of microanalysis and observation in microscopy and energy dispersive X-ray spectroscopy (EDXS) reveal a highly morphological diversity of MTB including unicellular cocci, vibrios, rod-shaped bacteria, and three morphotypes of multicellular magnetotactic prokaryotes (MMPs). In addition, analysis of the 16S rRNA gene showed that these MTB were clustered into 16 operational taxonomic units affiliated to the Alpha-, Delta-, and Gamma-proteobacteria classes within the Proteobacteria phylum. Meanwhile, by using the coupled fluorescence and transmission electron microscopy analysis, rodshaped bacteria, vibrio, and cocci were phylogenetically and structurally identified at the single-cell level. This study demonstrated highly diverse MTB communities in the mangrove ecosystem and provide a new insight into the overall diversity of MTB.
Key words:    magnetotactic bacteria|diversity|magnetosome|phylogenetic analysis|coordinated fluorescence in-situ hybridization transmission electron microscopy (FISH-TEM)|mangrove   
Received: 2020-12-30   Revised: 2021-05-06
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