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Lei ZHOU, Yaoquan HAN, Dapeng WANG, Yusen LI, Xiande HUANG, Anyou HE. Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1961-1971

Comparison of fungal community composition within different intestinal segments of tilapia and bighead carp

Lei ZHOU1,2, Yaoquan HAN1, Dapeng WANG1, Yusen LI1,2, Xiande HUANG2, Anyou HE1
1 Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China;
2 Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China
Although intestinal fungi play important roles in host health and disease, the composition and diversity of fungal communities remain poorly reported in fish. In this study, fungi in the fore-, mid-, and hindintestine of tilapia (Oreochromis mossambicus) and bighead carp (Aristichthys nobilis) from Hongchaojiang Reservoir in Guangxi, China were investigated by ITS sequencing. Based on this, we obtained 1 763 478 high-quality tags, which clustered into 1 089 operational taxonomic units (OTUs). In total, 404 OTUs were annotated, of which 310, 68, and 26 belonged to Ascomycota, Basidiomycota, and other, respectively. Results show significant differences in the community composition of intestinal fungi between tilapia and bighead carp but not within their different intestinal segments. Furthermore, 154 of the 404 annotated OTUs were considered reliable and were classified into three trophic modes and nine guilds. The three trophic modes consisted of 108 OTUs of saprotrophic fungi, 41 OTUs of pathotrophic fungi, and five OTUs of symbiotrophic fungi. The top three most abundant OTUs overall (i.e., Otu000002, Scopulariopsis acremonium; Otu000018, Alternaria palandui; Otu000034, Aureobasidium pullulans) showed lower abundance in the hind-intestinal segments of bighead carp, suggesting uneven distribution of these fungi in this species. In addition, saprotrophic and pathotrophic fungi were markedly decreased in the hindintestine. It is indicated that the fungal community was not only related to host species specificity but also to the respective physiological functions of different intestinal segments. These findings provide valuable information on the composition, structure, and potential function of the intestinal fungi community associated with different intestinal segments in tilapia and bighead carp under natural conditions, thus highlighting the importance of fungi as an integral part of the intestinal microbiota in maintaining host health.
Key words:    fungi|intestinal microbiome|tilapia|bighead carp|Internal Transcribed Spacer (ITS) sequencing   
Received: 2020-05-30   Revised: 2020-10-12
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