Cite this paper:
Weixiang LIU, Chaomin SUN. C17-fengycin B, produced by deep-sea-derived Bacillus subtilis, possessing a strong antifungal activity against Fusarium solani[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1938-1947

C17-fengycin B, produced by deep-sea-derived Bacillus subtilis, possessing a strong antifungal activity against Fusarium solani

Weixiang LIU1,2, Chaomin SUN1,2
1 CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean MegaScience, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
Root rot disease caused by Fusarium solani is the most devastating disease of the tomato and legume crops in China. The metabolites of Bacillus species can inhibit many fungal diseases. In this study, the metabolites of deep-sea-derived bacterium Bacillus subtilis 2H11 can significantly inhibit the growth of F. solani. The metabolite C17-fengycin B, one of the cyclic lipopeptides, was identified by the combination of silica column chromatography, high-performance liquid chromatography (HPLC), high-energy collision induced dissociation mass spectrometry (HCD-MS) and tandem mass spectrometry (HCD-MS/MS). The results of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that C17-fengycin B could destroy the structure of the hyphae and spores of F. solani. The antifungal activities of C17-fengycin B against F. solani were tested at concentrations ranging from 0.05 mg/mL to 0.20 mg/mL. The results indicated that C17-fengycin B inhibited the growth of F. solani with antifungal index of 89.80% at 0.20 mg/mL, and the antifungal activity of C17-fengycin B was further verified by the pot experiment. In addition, the cytotoxicity experiment showed that C17-fengycin B had good biocompatibility and was a potential candidate for the development of biocontrol pesticide in the future.
Key words:    Bacillus species|lipopeptide|fengycin|antifungal|pesticide   
Received: 2020-06-02   Revised: 2020-09-22
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