Cite this paper:
Junfeng SHI, Yanli LEI, Haotian LI, Tiegang LI. NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1718-1729

NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments

Junfeng SHI1,3,7, Yanli LEI1,4,5,6,7, Haotian LI1,6,7, Tiegang LI2
1 Laboratory of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Key Laboratory of Marine Sedimentology and Environmental Geology, First Institute of Oceanography, Ministry of Natural Resources (MNR), Qingdao 266061, China;
3 Clinical Research Center, Affiliated Hospital of Weifang Medical University, Weifang 261031, China;
4 Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, China;
5 Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China;
6 University of Chinese Academy of Sciences, Beijing 100049, China;
7 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Seamount is a unique deep-sea ecosystem widely distributed in the world. Its biodiversity is vibrant due to its specific geographical and hydrological conditions. However, the diversity and features of foraminifera in such an environment have rarely been studied. We extracted environmental DNA (eDNA) in sediments and amplified the partial small subunit ribosomal DNA (SSU rDNA) of foraminifera to understand the foraminiferal diversity from four sites in Magellan Seamount (Western Pacific Ocean). Partial SSU rDNA sequencing was conducted and 912 979 foraminiferal reads were obtained and gathered into 266 operational taxonomic units (OTUs). In the available dataset, a high proportion of rare OTUs and low identity OTUs in each studied sample showed that the Magellan Seamount foraminiferal community might have a high genetic novelty. The relative abundance of foraminifera varied between replicates probably due to the existence of bias in amplification process and patchiness of the deep-sea floor. It showed that the Magellan Seamount has a relatively high benthic foraminiferal diversity characterized mainly by monothalamiids (76.37% of total reads) in association with rotaliids (19.03% of total reads), including planktic foraminiferal sequences (38.58% of rotaliids; 7.36% of total reads). The remaining reads were assigned to miliolids (0.83% of total reads) and textulariids (0.66% of total reads), and 3.11% of total reads are unassigned to a specific family. The comparative analysis with foraminiferal assemblages from coastal and deep-sea environments indicated that seamounts could aggregate species from a nearby deep-sea.
Key words:    metabarcoding|Magellan Seamount|foraminifera|Western Pacific Ocean   
Received: 2020-11-11   Revised: 2021-01-14
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