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Qiqi SUN, Jinming SONG, Xuegang LI, Huamao YUAN, Qidong WANG. The bacterial diversity and community composition altered in the oxygen minimum zone of the Tropical Western Pacific Ocean[J]. Journal of Oceanology and Limnology, 2021, 39(5): 1690-1704

The bacterial diversity and community composition altered in the oxygen minimum zone of the Tropical Western Pacific Ocean

Qiqi SUN1,2,3, Jinming SONG2,3,4,5, Xuegang LI2,3,4,5, Huamao YUAN2,3,4,5, Qidong WANG2,3,4,5
1 Shandong Peanut Research Institute, Qingdao 266100, China;
2 Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
3 Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China;
4 University of Chinese Academy of Sciences, Beijing 100049, China;
5 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
The oxygen minimum zones (OMZs) are globally expanding, yet the variation pattern of microbial communities related to dissolved oxygen levels remain unclear. Spatial variability of bacterial diversity and community composition (represented by 16S rRNA) of six stations was investigated within the water column in the seamount area of Tropical Western Pacific Ocean (TWPO) in May 2019. The seawater has dissolved oxygen (DO) concentration of 3.01-6.68 mg/L and the core of the oxygen minimum zones was located between the depths of 650 m and 1 750 m. The bacterial alpha-diversity showed unimodal pattern with the decreasing DO with depths and peaked in the upper oxycline (UO) of OMZs. The bacterial community structure of the mixed layer (ML) and the bottom layer clustered and separated from each other, while those of UO and the OMZ core (OM) clustered and overlapped. Overall, bacterial community composition transitioned from being Alphaproteobacteria and Gammaproteobacteria-dominant in ML to being Gammaproteobacteria and Nitrososphaeria/Deltaproteobacteria-dominant in UO and OM, and then changed to being Clostridia and unidentified_Actinobacteria-dominant in the bottom layer. Moreover, both bacterial alpha-diversity and the abundant classes fitted varying sectioned functions with DO. The DO solely explained 40.37% of the variation of bacterial community composition among layers (P<0.001). The predicted function profiling showed that the water column was predominant by chemoheterotrophy, cyanobacteria, and photoautotrophy in ML, by chemoheterotrophy and nitrate/sulfide cycling in UO and OM, and by chemoheterotrophy and fermentation in the bottom layer. Our findings revealed the DO-associated variation in bacterial diversity and community composition, and help to clarify the potential responses of microbes and their involved biogeochemical processes to the expansion and intensification of OMZs.
Key words:    bacterial diversity|community composition|oxygen minimum zones (OMZs)|dissolved oxygen|Tropical Western Pacific Ocean (TWPO)   
Received: 2020-10-12   Revised: 2020-12-21
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Aldunate M, De La Iglesia R, Bertagnolli A D, Ulloa O. 2018. Oxygen modulates bacterial community composition in the coastal upwelling waters off central Chile. Deep Sea Research Part II:Topical Studies in Oceanography, 156:68-79,
Azam F, Malfatti F. 2007. Microbial structuring of marine ecosystems. Nature Reviews Microbiology, 5(10):782-791,
Baird D, Christian R R, Peterson C H, Johnson G A. 2004. Consequences of hypoxia on estuarine ecosystem function:energy diversion from consumers to microbes. Ecological Applications, 14(3):805-822,
Beman J M, Carolan M T. 2013. Deoxygenation alters bacterial diversity and community composition in the ocean's largest oxygen minimum zone. Nature Communications, 4:2705,
Bryant J A, Stewart F J, Eppley J M, DeLong E F. 2012. Microbial community phylogenetic and trait diversity declines with depth in a marine oxygen minimum zone. Ecology, 93(7):1 659-1 673,
Caporaso J G, Lauber C L, Walters W A, Berg-Lyons D, Lozupone C A, Turnbaugh P J, Fierer N, Knight R. 2011. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proceedings of the National Academy of Sciences of the United States of America, 108(S1):4 516-4 522,
Carolan M T, Smith J M, Beman J M. 2015. Transcriptomic evidence for microbial sulfur cycling in the eastern tropical north Pacific oxygen minimum zone. Frontiers in Microbiology, 6:334, 00334.
Dalsgaard T, Thamdrup B, Farías L, Revsbech N P. 2012. Anammox and denitrification in the oxygen minimum zone of the eastern south Pacific. Limnology and Oceanography, 57(5):1 331-1 346,
Desta A F, Assefa F, Leta S, Stomeo F, Wamalwa M, Njahira M, Appolinaire D. 2014. Microbial community structure and diversity in an integrated system of anaerobic-aerobic reactors and a constructed wetland for the treatment of tannery wastewater in Modjo, Ethiopia. PLoS One, 9(12):e115576,
Diaz R J, Rosenberg R. 2008. Spreading dead zones and consequences for marine ecosystems. Science, 321(5891):926-929,
Edgar R C. 2013. Uparse:highly accurate OTU sequences from microbial amplicon reads. Nature Methods, 10(10):996-998,
Fernandes G L, Shenoy B D, Damare S R. 2020. Diversity of bacterial community in the oxygen minimum zones of Arabian Sea and Bay of Bengal as deduced by Illumina sequencing. Frontiers in Microbiology, 10:3 153,
Gillies L E, Thrash J C, deRada S, Rabalais N N, Mason O U. 2015. Archaeal enrichment in the hypoxic zone in the northern Gulf of Mexico. Environmental Microbiology, 17(10):3 847-3 856,
Golyshina O V, Kublanov I V, Tran H, Korzhenkov A A, Lünsdorf H, Nechitaylo T Y, Gavrilov S N, Toshchakov S V, Golyshin P N. 2016. Biology of archaea from a novel family Cuniculiplasmataceae (Thermoplasmata) ubiquitous in hyperacidic environments. Scientific Reports, 6:39 034,
Gonsalves M J, Paropkari A L, Fernandes C E G, Bharathi P A L, Krishnakumari L, Fernando V, Nampoothiri G E. 2011. Predominance of anaerobic bacterial community over aerobic community contribute to intensify ‘oxygen minimum zone’ in the eastern Arabian Sea. Continental Shelf Research, 31(11):1 224-1 235, 1016/j.csr.2011.04.011.
Hawley A K, Brewer H M, Norbeck A D, Paša-Tolić L, Hallam S J. 2014. Metaproteomics reveals differential modes of metabolic coupling among ubiquitous oxygen minimum zone microbes. Proceedings of the National Academy of Sciences of the United States of America, 111(31):11 395-11 400,
Hoerling M P, Hurrell J W, Xu T Y. 2001. Tropical origins for recent north Atlantic climate change. Science, 292(5514):90-92,
Jain A, Bandekar M, Gomes J, Shenoy D, Meena R M, Naik H, Khandeparkar R, Ramaiah N. 2014. Temporally invariable bacterial community structure in the Arabian Sea oxygen minimum zone. Aquatic Microbial Ecology, 73(1):51-67,
Jayakumar A, O'Mullan G D, Naqvi S W A, Ward B B. 2009. Denitrifying bacterial community composition changes associated with stages of denitrification in oxygen minimum zones. Microbial Ecology, 58(2):350-362,
Kang I, Vergin K L, Oh H M, Choi A, Giovannoni S J, Cho J C. 2011. Genome sequence of strain HTCC2083, a novel member of the marine clade Roseobacter. Journal of Bacteriology, 193(1):319-320,
Li X G, Song J M, Yuan H M, Li N, Duan L Q, Wang Q D. 2017. The oxygen minimum zones (OMZs) and its ecoenvironmental effects in ocean. Marine Sciences, 41(12):127-138, (in Chinese with English abstract)
Ma J, Song J M, Li X G, Yuan H M, Li N, Duan L Q, Wang Q D. 2019. Environmental characteristics in three seamount areas of the tropical western Pacific Ocean:focusing on nutrients. Marine Pollution Bulletin, 143:163-174,
Ma J, Song J M, Li X G, Yuan H M, Li N, Duan L Q, Wang Q D. 2020. Control factors of DIC in the Y3 seamount waters of the Western Pacific Ocean. Journal of Oceanology and Limnology, 38(C7):1 215-1 224,
Muck S, De Corte D, Clifford E L, Bayer B, Herndl G J, Sintes E. 2019. Niche differentiation of aerobic and anaerobic ammonia oxidizers in a high latitude deep oxygen minimum zone. Frontiers in Microbiology, 10:2 141,
Naqvi S W A. 1994. Denitrification processes in the Arabian Sea. Proceedings of the Indian Academy of SciencesEarth and Planetary Sciences, 103(2):279-300,
Pajares S, Varona-Cordero F, Hernández-Becerril D U. 2020. Spatial distribution patterns of bacterioplankton in the oxygen minimum zone of the tropical Mexican Pacific. Microbial Ecology, 80(3):519-536,
Paulmier A, Ruiz-Pino D, Garçon V. 2011. CO2 maximum in the oxygen minimum zone (OMZ). Biogeosciences, 8(2):239-252,
Paulmier A, Ruiz-Pino D. 2009. Oxygen minimum zones(OMZs) in the modern ocean. Progress in Oceanography, 80(3-4):113-128, 08.001.
R Core Team. 2013. R:A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria.
Rissanen A J, Saarenheimo J, Tiirola M, Peura S, Aalto S L, Karvinen A, Nykänen H. 2018. Gammaproteobacterial methanotrophs dominate methanotrophy in aerobic and anaerobic layers of boreal lake waters. Aquatic Microbial Ecology, 81(3):257-276,
Spietz R L, Williams C M, Rocap G, Horner-Devine M C. 2015. A dissolved oxygen threshold for shifts in bacterial community structure in a seasonally hypoxic estuary. PLoS One, 10(8):e0135731,
Stevens H, Ulloa O. 2008. Bacterial diversity in the oxygen minimum zone of the eastern tropical south Pacific. Environmental Microbiology, 10(5):1 244-1 259,
Sun Q Q, Song J M, Li X G, Yuan H M, Ma J, Wang Q D. 2020. Bacterial vertical and horizontal variability around a deep seamount in the tropical western Pacific Ocean. Marine Pollution Bulletin, 158:111 419,
Thomas Y, Flye-Sainte-Marie J, Chabot D, Aguirre-Velarde A, Marques G, Pecquerie L. 2019. Effects of hypoxia on metabolic functions in marine organisms:observed patterns and modelling assumptions within the context of dynamic energy budget (DEB) theory. Journal of Sea Research, 143:231-242,
Tian D F, Li X G, Song J M, Li N. 2019. Process and mechanism of nitrogen loss in the ocean oxygen minimum zone. Chinese Journal of Applied Ecology, 30(3):1 047-1 056, (in Chinese with English abstract).
Tian D F, Wang Y Q, Xing J W, Sun Q Q, Song J M, Li X G. 2020. Nitrogen loss process in hypoxic seawater based on the culture experiment. Marine Pollution Bulletin, 152:110 912,
Ulloa O, Canfield D E, Delong E F, Letelier R M, Stewart F J. 2012. Microbial oceanography of anoxic oxygen minimum zones. Proceedings of the National Academy of Sciences of the United States of America, 109(40):15 996-16 003,
Ulloa O, Wright J J, Belmar L, Hallam S J. 2013. Pelagic oxygen minimum zone microbial communities. In:Rosenberg E, DeLong E F, Lory S, Stackebrandt E, Thompson F eds. The Prokaryotes. Springer, Berlin, Heidelberg, p.113-122,
Walsh D A, Zaikova E, Howes C G, Song Y C, Wright J J, Tringe S G, Tortell P D, Hallam S J. 2009. Metagenome of a versatile chemolithoautotroph from expanding oceanic dead zones. Science, 326(5952):578-582,
Wang Q, Garrity G M, Tiedje J M, Cole J R. 2007. Naïve Bayesian classifier for rapid assignment of RRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology, 73(16):5 261-5 267,
Wishner K F, Outram D M, Seibel B A, Daly K L, Williams R L. 2013. Zooplankton in the eastern tropical north Pacific:boundary effects of oxygen minimum zone expansion. Deep Sea Research Part I:Oceanographic Research Papers, 79:122-140, 05.012.
Wright J J, Konwar K M, Hallam S J. 2012. Microbial ecology of expanding oxygen minimum zones. Nature Reviews Microbiology, 10(6):381-394,
Zhang W, Liu J, Dong Y et al. 2019. Archaeal community structure in sediments from a seamount in the Mariana volcanic arc. Journal of Oceanology and Limnology, 37(4):1 197-1 210,
Zhong H H, Lehtovirta-Morley L, Liu J W, Zheng Y F, Lin H Y, Song D L, Todd J D, Tian J W, Zhang X H. 2020. Novel insights into the Thaumarchaeota in the deepest oceans:Their metabolism and potential adaptation mechanisms. Microbiome, 8(1):78,
Zuo J L, Song J M, Yuan H M, Li X G, Duan L Q. 2019. Impact of Kuroshio on the dissolved oxygen in the East China Sea region. Journal of Oceanology and Limnology, 37(2):513-524,
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