Cite this paper:
Jingjing SONG, Haiyi WU, Kaikai LIU, Wendan CHI, Shanshan GE, Zhipeng ZHANG, Mingqi WANG, Yi YU, Daode YU. Observation and analysis of morphology abnormalities in development of Oryzias melastigma embryos[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1485-1499

Observation and analysis of morphology abnormalities in development of Oryzias melastigma embryos

Jingjing SONG1, Haiyi WU1, Kaikai LIU1, Wendan CHI1, Shanshan GE1, Zhipeng ZHANG2, Mingqi WANG2, Yi YU3, Daode YU1
1 Healthy Mariculture Engineering Research Center of Shandong Province, Marine Science Research Institute of Shandong Province, Qingdao 266104, China;
2 Tianjin Research Institute for Water Transport Engineering, Ministry of Transport, Tianjin 300456, China;
3 Ocean University of China, Qingdao 266100, China
Fish embryos are widely used as models in toxicology, drug development, and human disease research because of their high sensitivity, observability, and operability, providing the basis for an in-depth understanding of the embryogenesis. Increasing studies have indicated that birth defects are hereditary. In this study, we used Oryzias melastigma as a model to conduct a study of 185-day embryogenesis and observed self-induced non-pathological abnormal embryogenesis. O. melastigma experienced pre-puberty stage, adolescence stage, and senescence stage, and individuals produced up to 102 eggs per day. However, the fecundity was markedly reduced during the senescent stage. During the active egg and blastodisc stages, pseudo-fertilization and pseudo-blastocysts were observed. During cleavage at the 4-to 32-cell stages, we observed blastomeres separation or dislocation. Excessively separated blastomeres formed double blastoderms, eventually resulting in conjoined twins. During the blastula stage, we observed abnormally increased cell volume, narrowed and elongated blastocysts, and abnormally coated blastoderms. At the organogenesis stage, we observed abnormal numbers of Kupffer's vesicles and conjoined twins. Abnormality in the location and number of oil droplets were observed in various development stages. Abnormal development was more commonly observed in fertilized eggs produced by broodstock in prepuberty or senescence stages, which is probably related to the age of fish and the egg quality. This study can provide the materials for comparative analysis in toxicological and molecular studies of O. melastigma, and may provide evidence for other economic fish that produce sticky eggs.
Key words:    marine fish model|abnormal embryogenesis|blastomeres separation|Kupffer's vesicle|conjoined twins   
Received: 2020-06-10   Revised: 2020-08-02
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