Cite this paper:
Shujin GUO, Zengxia ZHAO, Junhua LIANG, Juan DU, Xiaoxia SUN. Carbon biomass, carbon-to-chlorophyll a ratio and the growth rate of phytoplankton in Jiaozhou Bay, China[J]. Journal of Oceanology and Limnology, 2021, 39(4): 1328-1342

Carbon biomass, carbon-to-chlorophyll a ratio and the growth rate of phytoplankton in Jiaozhou Bay, China

Shujin GUO1,2,3, Zengxia ZHAO1,2,3, Junhua LIANG1,2,3, Juan DU1,2,3, Xiaoxia SUN1,2,3,4
1 Jiaozhou Bay National Marine Ecosystem Research Station, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;
3 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
4 University of Chinese Academy of Sciences, Beijing 100049, China
Carbon biomass, carbon-to-chlorophyll a ratio (C:Chl a), and the growth rate of phytoplankton cells were studied during four seasonal cruises in 2017 and 2018 in Jiaozhou Bay, China. Water samples were collected from 12 stations, and phytoplankton carbon biomass (phyto-C) was estimated from microscope-measured cell volumes. The phyto-C ranged from 5.05 to 78.52 μg C/L in the bay, and it constituted a mean of 38.16% of the total particulate organic carbon in the bay. High phyto-C values appeared mostly in the northern or northeastern bay. Diatom carbon was predominant during all four cruises. Dinoflagellate carbon contributed much less (<30%) to the total phyto-C, and high values appeared often in the outer bay. The C:Chl a of phytoplankton cells varied from 11.50 to 61.45 (mean 31.66), and high values appeared in the outer bay during all four seasons. The phyto-C was also used to calculate the intrinsic growth rates of phytoplankton cells in the bay, and phytoplankton growth rates ranged from 0.56 to 1.96/d; the rate was highest in summer (mean 1.79/d), followed by that in fall (mean 1.24/d) and spring (mean 1.17/d), and the rate was lowest in winter (mean 0.77/d). Temperature and silicate concentration were found to be the determining factors of phytoplankton growth rates in the bay. To our knowledge, this study is the first report on phytoplankton carbon biomass and C:Chl a based on water samples in Jiaozhou Bay, and it will provide useful information for studies on carbon-based food web calculations and carbon-based ecosystem models in the bay.
Key words:    phytoplankton|carbon biomass|carbon-to-chlorophyll a (C:Chl a) ratio|growth rates|Jiaozhou Bay   
Received: 2020-06-17   Revised: 2020-07-29
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Ara K, Fukuyama S, Okutsu T, Nagasaka S, Shiomoto A. 2019. Seasonal variability in phytoplankton carbon biomass and primary production, and their contribution to particulate carbon in the neritic area of Sagami Bay, Japan. Plankton and Benthos Research, 14(4):224-250.
Arteaga L, Pahlow M, Oschlies A. 2016. Modeled Chl:C ratio and derived estimates of phytoplankton carbon biomass and its contribution to total particulate organic carbon in the global surface ocean. Global Biogeochemical Cycles, 30(12):1 791-1 810.
Boyd P W, Rynearson T A, Armstrong E A, Fu F X, Hayashi K, Hu Z X, Hutchins D A, Kudela R M, Litchman E, Mulholland M R, Passow U, Strzepek R F, Whittaker K A, Yu E, Thomas M K. 2013. Marine phytoplankton temperature versus growth responses from polar to tropical waters-outcome of a scientific community-wide study. PLoS One, 8(5):e63091.
Chang F H, Zeldis J, Gall M, Hall J. 2003a. Seasonal and spatial variation of phytoplankton assemblages, biomass and cell size from spring to summer across the north-eastern New Zealand continental shelf. Journal of Plankton Research, 25(7):737-758.
Chang J, Shiah F K, Gong G C, Chiang K P. 2003b. Cross-shelf variation in carbon-to-chlorophyll a ratios in the East China Sea, summer 1998. Deep Sea Research Part II:Topical Studies in Oceanography, 50(6-7):1 237-1 247.
Cloern J E, Grenz C, Vidergar-Lucas L. 1995. An empirical model of the phytoplankton chlorophyll:carbon ratio-the conversion factor between productivity and growth rate.
Limnology and Oceanography, 40(7):1 313-1 321.
Crawford D W, Cefarelli A O, Wrohan I A, Wyatt S N, Varela D E. 2018. Spatial patterns in abundance, taxonomic composition and carbon biomass of nano- and microphytoplankton in Subarctic and Arctic Seas.Progress in Oceanography, 162:132-159.
Eppley R W. 1972. Temperature and phytoplankton growth in the sea. Fishery Bulletin, 70(4):1 063-1 085.
Falkowski P G, Owens T G. 1980. Light-shade adaptation two strategies in marine phytoplankton. Plant Physiology, 66(4):592-595.
Friedland K D, Stock C, Drinkwater K F, Link J S, Leaf R T, Shank B V, Rose J M, Pilskaln C H, Fogarty M J. 2012.Pathways between primary production and fisheries yields of large marine ecosystems. PLoS One, 7(1):e28945.
Fu M Z, Wang Z L, Li Y, Li R X, Sun P, Wei X H, Lin X Z, Guo J S. 2009. Phytoplankton biomass size structure and its regulation in the Southern Yellow Sea (China):seasonal variability. Continental Shelf Research, 29(18):2 178-2 194.
Geider R J. 1987. Light and temperature dependence of the carbon to chlorophyll a ratio in microalgae and cyanobacteria:implications for physiology and growth of phytoplankton. New Phytologist, 106(1):1-34.
Gong G C, Chen Y L L, Liu K K. 1996. Chemical hydrography and chlorophyll a distribution in the East China Sea in summer:implications in nutrient dynamics. Continental Shelf Research, 16(12):1 561-1 590.
Graff J R, Milligan A J, Behrenfeld M J. 2012. The measurement of phytoplankton biomass using flow-cytometric sorting and elemental analysis of carbon. Limnology and Oceanography:Methods, 10(11):910-920.
Graff J R, Westberry T K, Milligan A J, Brown M B, Dall'Olmo G, van Dongen-Vogels V, Reifel K M, Behrenfeld M J. 2015. Analytical phytoplankton carbon measurements spanning diverse ecosystems. Deep Sea Research Part I:Oceanographic Research Papers, 102:16-25.
Guo S J, Feng Y Y, Wang L, Dai M H, Liu Z L, Bai Y, Sun J. 2014. Seasonal variation in the phytoplankton community of a continental-shelf sea:the East China Sea. Marine Ecology Progress Series, 516:103-126.
Guo S J, Zhu M L, Zhao Z X, Liang J H, Zhao Y F, Du J, Sun X X. 2019. Spatial-temporal variation of phytoplankton community structure in Jiaozhou Bay, China. Journal of Oceanology and Limnology, 37(5):1 611-1 624.
Harrison P J, Zingone A, Mickelson M J, Lehtinen S, Ramaiah N, Kraberg A C, Sun J, McQuatters-Gollop A, Jakobsen H H. 2015. Cell volumes of marine phytoplankton from globally distributed coastal data sets. Estuarine, Coastal and Shelf Science, 162:130-142.
Hillebrand H, Dürselen C D, Kirschtel D, Pollingher U, Zohary T. 1999. Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology, 35(2):403-424.
Jakobsen H H, Carstensen J. 2011. FlowCAM:sizing cells and understanding the impact of size distributions on biovolume of planktonic community structure. Aquatic Microbial Ecology, 65(1):75-87.
Jakobsen H H, Markager S. 2016. Carbon-to-chlorophyll ratio for phytoplankton in temperate coastal waters:seasonal patterns and relationship to nutrients. Limnology and Oceanography, 61(5):1 853-1 868.
Jeong H J, Yoo Y D, Kim J S, Seong K A, Kang N S, Kim T H. 2010. Growth, feeding and ecological roles of the mixotrophic and heterotrophic dinoflagellates in marine planktonic food webs. Ocean Science Journal, 45(2):65-91.
Kopczyńska E E, Fiala M. 2003. Surface phytoplankton composition and carbon biomass distribution in the Crozet Basin during austral summer of 1999:variability across frontal zones. Polar Biology, 27(1):17-28.
Kruskopf M, Flynn K J. 2006. Chlorophyll content and fluorescence responses cannot be used to gauge reliably phytoplankton biomass, nutrient status or growth rate.New Phytologist, 169(3):525-536.
Legendre L, Michaud J. 1999. Chlorophyll a to estimate the particulate organic carbon available as food to large zooplankton in the euphotic zone of oceans. Journal of Plankton Research, 21(11):2 067-2 083.
Li Q P, Franks P J S, Landry M R, Goericke R, Taylor A G. 2010. Modeling phytoplankton growth rates and chlorophyll to carbon ratios in California coastal and pelagic ecosystems. Journal of Geophysical Research:Biogeosciences, 115(G4):G04003.
Liu D Y, Sun J, Qian S B. 2002. Study on the phytoplankton in Jiaozhou Bay II:influence of the environmental factors to phytoplankton community. Journal of Ocean University of Qingdao, 32(3):415-421. (in Chinese with English abstract)
Liu X, Huang B Q, Huang Q, Wang L, Ni X B, Tang Q S, Sun S, Wei H, Liu S M, Li C L, Sun J. 2015. Seasonal phytoplankton response to physical processes in the southern Yellow Sea. Journal of Sea Research, 95:45-55.
Lü S G, Wang X C, Han B P. 2009. A field study on the conversion ratio of phytoplankton biomass carbon to chlorophyll-a in Jiaozhou Bay, China. Chinese Journal of Oceanology and Limnology volume, 27(4):793-805.
Marañón E, Holligan P M, Varela M, Mouriño B, Bale A J. 2000. Basin-scale variability of phytoplankton biomass, production and growth in the Atlantic Ocean. Deep Sea Research Part I:Oceanographic Research Papers, 47(5):825-857.
Menden-Deuer S, Lessard E J. 2000. Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnology and Oceanography, 45(3):569-579.
Örnólfsdóttir E B, Lumsden S E, Pinckney J L. 2004.Phytoplankton community growth-rate response to nutrient pulses in a shallow turbid estuary, Galveston Bay, Texas. Journal of Plankton Research, 26(3):325-339.
Pinckney J L, Richardson T L, Millie D F, Paerl H W. 2001.Application of photopigment biomarkers for quantifying microalgal community composition and in situ growth rates. Organic Geochemistry, 32(4):585-595.
Putland J N, Iverson R L. 2007. Phytoplankton biomass in a subtropical estuary:distribution, size composition, and carbon:chlorophyll ratios. Estuaries and Coasts, 30(5):878-885.
Regaudie-de-Gioux A, Sal S, López-Urrutia Á. 2015. Poor correlation between phytoplankton community growth rates and nutrient concentration in the sea. Biogeosciences, 12(6):1 915-1 923.
Sathyendranath S, Stuart V, Nair A, Oka K, Nakane T, Bouman H, Forget M H, Maass H, Platt T. 2009. Carbon-to-chlorophyll ratio and growth rate of phytoplankton in the sea. Marine Ecology Progress Series, 383:73-84.
Sherman E, Moore J K, Primeau F, Tanouye D. 2015.Temperature influence on phytoplankton community growth rates. Global Biogeochemical Cycles, 30(4):550-559.
Shi X Y, Wang L S, Yang S M. 2015. Phytoplankton community of Jiaozhou Bay in winter 2010. Oceanologia et Limnologia Sinica, 46(2):357-364. (in Chinese with English abstract)
Shinada A, Ban S, Ikeda T. 2008. Seasonal changes in the planktonic food web off Cape Esan, southwestern Hokkaido, Japan. Plankton and Benthos Research(Japan), 3(1):18-26.
Smith Jr W O, Nelson D M, Mathot S. 1999. Phytoplankton growth rates in the Ross Sea, Antarctica, determined by independent methods:temporal variations. Journal of Plankton Research, 21(8):1 519-1 536.
Spaulding S A, Jewson D H, Bixby R J, Nelson H, McKnight D M. 2012. Automated measurement of diatom size.Limnology and Oceanography:Methods, 10(11):882-890.
Stel'makh L V, Babich I I, Tugrul S, Moncheva S, Stefanova K. 2009. Phytoplankton growth rate and zooplankton grazing in the western part of the Black Sea in the autumn period. Oceanology, 49(1):83-92.
Sun J, Liu D Y, Qian S B. 1999. Study on phytoplankton biomass I. Phytoplankton measurement biomass from cell volume or plasma volume. Acta Oceanologica Sinica, 21(2):75-85. (in Chinese with English abstract)
Sun J, Liu D Y, Qian S B. 2000. Estimating biomass of phytoplankton in the Jiaozhou Bay I. Phytoplankton biomass estimated from cell volume and plasma volume.Acta Oceanologica Sinica, 19(2):97-110.
Sun J, Liu D Y. 2003. Geometric models for calculating cell biovolume and surface area for phytoplankton. Journal of Plankton Research, 25(11):1 331-1 346.
Sun X X, Sun S, Wu Y L, Zhang Y S, Zheng S. 2011a. Long-term changes of phytoplankton community structure in the Jiaozhou Bay. Oceanologia et Limnologia Sinica, 42(5):639-646. (in Chinese with English abstract)
Sun X X, Sun S, Zhang Y S, Zhang F. 2011b. Long-term changes of chlorophyll-a concentration and primary productivity in the Jiaozhou Bay. Oceanologia et Limnologia Sinica, 42(5):654-661. (in Chinese with English abstract)
Sun X X, Sun S. 2012. Phytoplankton size structure and its temporal and spatial changes in Jiaozhou Bay.Oceanologia et Limnologia Sinica, 43(3):411-418. (in Chinese with English abstract)
Tan S J. 2009. Preliminary Studies on Cascade Grazing of Mesozooplankton on Phytoplankton and Microzooplankton Community in the Jiaozhou Bay.Ocean University of China, Qingdao. (in Chinese)
Utermöhl H. 1958. Zur vervollkommnung der quantitativen phytoplankton-methodik. SIL Communications, 1953-1996, 9(1):1-38.
Wang X J, Borgne R L, Murtugudde R, Busalacchi A J, Behrenfeld M. 2009. Spatial and temporal variability of the phytoplankton carbon to chlorophyll ratio in the equatorial Pacific:a basin-scale modeling study. Journal of Geophysical Research, 114(C7):C07008.
Wu Y L, Sun S, Zhang Y S. 2005. Long-term change of environment and it's influence on phytoplankton community structure in Jiaozhou Bay. Oceanologia et Limnologia Sinica, 36(6):487-498. (in Chinese with English abstract)
Xiao W P, Liu X, Irwin A J, Laws E A, Wang L, Chen B Z, Zeng Y, Huang B Q. 2018. Warming and eutrophication combine to restructure diatoms and dinoflagellates. Water Research, 128:206-216.
Yang D F, Gao Z H, Sun P Y, Zhao B, Li M. 2006. Spatial and temporal variations of the primary production limited by nutrient silicon and water temperature in the Jiaozhou Bay. Advances in Marine Science, 24(2):203-212. (in Chinese with English abstract)
Yang S M, Wang L S, Shi X Y. 2014. Phytoplankton community of the Jiaozhou Bay in spring 2009. Oceanologia et Limnologia Sinica, 45(6):1 234-1 240. (in Chinese with English abstract)
Yang Y, Sun X X, Zhu M L, Luo X, Zheng S. 2017. Estimating the carbon biomass of marine net phytoplankton from abundance based on samples from China seas. Marine and Freshwater Research, 68(1):106-115.
Yao Y, Zheng S Q, Shen Z L. 2007. Study on the mechanism of eutrophication in the Jiaozhou Bay. Marine Science Bulletin, 26(4):91-98. (in Chinese with English abstract)
Zhou W H, Yin K D, Long A M, Huang H, Huang L M, Zhu D D. 2012. Spatial-temporal variability of total and size-fractionated phytoplankton biomass in the Yangtze River Estuary and adjacent East China Sea coastal waters, China. Aquatic Ecosystem Health & Management, 15(2):200-209.
Zhou Y P, Zhang Y M, Li F F, Tan L J, Wang J T. 2017.Nutrients structure changes impact the competition and succession between diatom and dinoflagellate in the East China Sea. Science of the Total Environment, 574:499-508.
Zonneveld C. 1998. A cell-based model for the chlorophyll a to carbon ratio in phytoplankton. Ecological Modelling, 113(1-3):55-70.
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