Cite this paper:
Zishuo CHEN, Tao LI, Bingjie YANG, Xuejie JIN, Hualian WU, Jiayi WU, Yandu LU, Wenzhou XIANG. Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1131-1142

Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield

Zishuo CHEN1,4, Tao LI1,3, Bingjie YANG1,4, Xuejie JIN1,3, Hualian WU1,3, Jiayi WU1,3, Yandu LU2, Wenzhou XIANG1,3
1 CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
2 State Key Laboratory of Marine Resource Utilization in South China Sea, College of Oceanology, Hainan University, Haikou 570228, China;
3 Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou), Guangzhou 511458, China;
4 University of Chinese Academy of Sciences, Beijing 100049, China
The use of high alkaline medium is a feasible way to provide carbon source and prevent biological contamination for the outdoor cultivation of alkaliphilic microalgae and cyanobacteria. A novel cyanobacterial strain was isolated from the open pond of a marine green alga (Picochlorum sp. SCSIO-45015, Sanya, Hainan) and identified as Cyanobacterium sp. SCSIO-45682. The effects of initial sodium bicarbonate (NaHCO3) concentrations on the growth and biochemical composition of Cyanobacterium sp. SCSIO-45682 were investigated. The results demonstrated that Cyanobacterium sp. SCSIO-45682 had good adaptation to 16.8-g/L NaHCO3 (the same concentration of NaHCO3 used in Zarrouk medium for Spirulina). Moreover, the yields of biomass, polysaccharide, chlorophyll a (chl a), and phycocyanin increased under high NaHCO3 concentrations. The maximum final biomass concentration of 2.5 g/L was observed at 8.4-g/L NaHCO3, while the highest intracellular total saccharide content of 49.2% of dry weight (DW) and exopolysaccharide (EPS) concentration of 93 mg/L were achieved at the NaHCO3 concentration of 16.8 g/L. The crude protein content declined under high NaHCO3 concentrations, which provide a possible explanation for the accumulation of polysaccharide. This study shows a good potential of alkaliphilic Cyanobacterium sp. SCSIO-45682 as a polysaccharide feedstock.
Key words:    alkaliphilic cyanobacterium|biochemical composition|Cyanobacterium sp. SCSIO-45682|high sodium bicarbonate (NaHCO3) concentrations|polysaccharide   
Received: 2020-03-05   Revised: 2020-04-22
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