Cite this paper:
Yanhong SUN, Pei LI, Guiying WANG, Renli SUN, Jian CHEN, Qi ZHOU, Jingou TONG, Qing LI. Construction of the first high-density genetic map for growth related QTL analysis in Ancherythroculter nigrocauda[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1118-1130

Construction of the first high-density genetic map for growth related QTL analysis in Ancherythroculter nigrocauda

Yanhong SUN1,2, Pei LI1,2, Guiying WANG1,2, Renli SUN1,2, Jian CHEN1,2, Qi ZHOU3, Jingou TONG4, Qing LI1,2
1 Fisheries Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan 430207, China;
2 Wuhan Xianfeng Aquaculture Technology Co. Ltd., Wuhan 430207, China;
3 Wuhan Academy of Agricultural Sciences, Wuhan 430207, China;
4 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Ancherythroculter nigrocauda is a fish endemic to the upper areas of the Changjiang (Yangtze) River in China. Quantitative trait locus (QTL) mapping is a powerful tool to identify potential genes affecting traits of economic importance in domestic animals. In this study, a high-density genetic map was constructed with 5 901 single nucleotide polymorphism (SNP) makers by sequencing 92 individual fish from a F1 family using the specific-locus amplified fragment sequencing approach. Initially, 48 QTLs for total length, body length, body height, and body weight were identified according to the high density of the genetic map with 24 LGs, a total length of 3 839.4 cM, and marker spacing of about 0.82 cM. These QTLs explained 27.1%-49.9% of phenotypic variance. The results of this study suggest that major QTLs are responsible for the growth of A. nigrocauda, and these are potentially useful in comparative genomics research, genome assembly, and marker-assisted breeding programs for this species.
Key words:    Ancherythroculter nigrocauda|specific-locus amplified fragment|high-density genetic map|quantitative trait locus   
Received: 2019-11-20   Revised: 2020-01-26
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