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Huaqiang TAN, Wenhua LIU, Ping LI. Taxonomic reassessment of Polysiphonia hainanensis (Rhodomelaceae, Rhodophyta) based on molecular and morphological analyses[J]. Journal of Oceanology and Limnology, 2021, 39(3): 1077-1084

Taxonomic reassessment of Polysiphonia hainanensis (Rhodomelaceae, Rhodophyta) based on molecular and morphological analyses

Huaqiang TAN1,2, Wenhua LIU1,2, Ping LI1,2
1 Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou 515063, China;
2 Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou), Guangzhou 511458, China
Polysiphonia hainanensis was previously described from Hainan, China, but DNA sequence had never been provided and its phylogenetic position in Polysiphonieae has not been clear so far. The classification of Polysiphonieae has changed recently based on the molecular analyses and detail morphological anatomy, consequently, the generic assignment of some species must be reassessed. In this study, we analyzed phylogenetic relationships of P. hainanensis and the related taxa in Polysiphonieae and Streblocladieae based on rbcL sequences, with specimens collected from the type locality and the area around. Two genetic types were detected, and formed a clade with Bryocladia thyrsigera with strong bootstrap support, in which the divergence was less than 0.81%. Furthermore, based on morphological analyses, we found characteristics of Chinese specimens, number of pericentral cells, percurrent axis, determinate branchlets, branching pattern, spermatangial axes development, and arrangement of tetrasporangia, etc., were consistent with those of Bryocladia. Combined with morphological and molecular analyses, P. hainanensis was taken a synonymous of B. thyrsigera, and gametophytes of this species were found from China for the first time.
Key words:    Bryocladia|phylogeny|Polysiphonieae|rbcL   
Received: 2020-02-19   Revised: 2020-03-29
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Agardh J G. 1847. Nya alger från Mexico. Öfversigt af Kongl.(Svenska) Vetenskaps-Akademiens Förhandlingar, 4:5-17.
Dawes C J, Mathieson A C. 2008. The Seaweeds of Florida.University Press of Florida, Gainesville, Florid. 592p.
De Toni G B. 1903. Sylloge Algarum Omnium Hucusque Cognitarum. Vol. IV. Florideae. Sectio Ⅲ. Sumptibus auctoris, Patavii, Padua, Italy. p.775-777.
Díaz-Tapia P, Maggs C A, West J A, Verbruggen H. 2017a.Analysis of chloroplast genomes and a supermatrix inform reclassification of the Rhodomelaceae(Rhodophyta). Journal of Phycology, 53(5):920-937,
Díaz-Tapia P, McIvor L, Freshwater D W, Verbruggen H, Wynne M J, Maggs C A. 2017b. The genera Melanothamnus Bornet & Falkenberg and Vertebrata S.F.Gray constitute well-defined clades of the red algal tribe Polysiphonieae (Rhodomelaceae, Ceramiales). European Journal of Phycology, 52(1):1-30, 0/09670262.2016.1256436.
Díaz-Tapia P, Pasella M, Verbruggen H. 2018. Molecular analyses resolve the phylogenetic position of Polysiphonia adamsiae (Rhodomelaceae, Rhodophyta) and reveal a strong phylogeographic structure in Australia. Phycologia, 57(5):593-600,
Falkenberg P. 1901. Die Rhodomelaceen des Golfes von Neapel und der Angrenzenden Meeres-Abschnitte. R.Friedländer and Sons, Berlin, Germany. p.1-754.
Guindon S, Gascuel O. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52(5):696-704,
Guiry M D. 2020. AlgaeBase is a global algal database of taxonomic, nomenclatural and distributional information. Accessed on 2020-04-28.
Huisman J M, Kim B, Kim M S. 2017. The phylogenetic position of Polysiphonia scopulorum (Rhodomelaceae, Rhodophyta) based on molecular analyses and morphological observations of specimens from the type locality in Western Australia. Phytotaxa, 324(1):51,
Kim M S, Yang E C, Mansilla A, Boo S M. 2004. Recent introduction of Polysiphonia morrowii (Ceramiales, Rhodophyta) to Punta Arenas, Chile. Botanica Marina, 47(5):389-394,
Kim M S, Yang E C. 2005. Taxonomic note of Polysiphonia pacifica (Ceramiales, Rhodophyta) complex with focus on Pacific isolates. ALGAE, 20(1):15-23,
Larkin M A, Blackshields G, Brown N P, Chenna R, McGettigan P A, McWilliam H, Valentin F, Wallace I M, Wilm A, Lopez R, Thompson J D, Gibson T J, Higgins D G. 2007. Clustal W and Clustal X version 2.0.Bioinformatics, 23(21):2 947-2 948,
Mamoozadeh N R, Freshwater D W. 2012. Polysiphonia sensu lato (Ceramiales, Florideophyceae) species of Caribbean Panama including Polysiphonia lobophoralis sp. nov. and Polysiphonia nuda sp. nov. Botanica Marina, 55(4):317-347,
McIvor L, Maggs C A, Provan J, Stanhope M J. 2001. RbcL sequences reveal multiple cryptic introductions of the Japanese red alga Polysiphonia harveyi. Molecular Ecology, 10(4):911-919,
Posada D, Crandall K A. 1998. Modeltest:testing the model of DNA substitution. Bioinformatics, 14(9):817-818,
Ronquist F, Teslenko M, van der Mark P, Ayres D L, Darling A, Höhna S, Larget B, Liu L, Suchard M A, Huelsenbeck J P. 2012. MrBayes 3.2:efficient Bayesian phylogenetic inference and model choice across a large model space.Systematic Biology, 61(3):539-542,
Schmitz F, Falkenberg P. 1897. Rhodomelaceae. In:Engler A, Prantt K eds. Die Natürlichen Pflanzenfamilien Nebst Ihren Gattungen und Wichtigeren Arten Insbesondere den Nutzpflanzen unter Mitwirkung Zahlreicher Hervorragender Fachgelehrten, Teil 1, Abteilung 2. Verlag Von Wilhelm Engelmann, Leipzig. p.421-480.
Stuercke B, Freshwater D W. 2008. Consistency of morphological characters used to delimit Polysiphonia sensu lato species (Ceramiales, Florideophyceae):analyses of North Carolina, USA specimens. Phycologia, 47(6):541-559,
Suárez A M, Alfonso Y, Martínez Daranas B R. 2015.Macroalgas Marinas de Cuba. Editorial UH, La Habana. 264p.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5:molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10):2 731-2 739,
Womersley H B S. 1979. Southern Australian species of Polysiphonia Greville (Rhodophyta). Australian Journal of Botany, 27(4):459-528,
Xia B M, Wang Y Q. 2012. A new species of Polysiphonia(Rhodophyta) from China. Oceanologia et Limnologia Sinica, 43(5):963-966. (in Chinese with English abstract)
Xia B M. 2011. Flora Algarum Marinarum Sinicarum Tomus Ⅱ Rhodophyta No. VⅡ Ceramiales Rhodomelaceae. Science Press, Beijing, China. 212p. (in Chinese)
Xiang S D. 2004. Study on Polysiphonia and Neosiphonia in China. Journal of Zhejiang University (Science Edition), 31(1):88-97. (in Chinese with English abstract)
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