Cite this paper:
Xiaoyong ZHANG, Yiyang LI, Zonghe YU, Xiao LIANG, Shuhua QI. Phylogenetic diversity and bioactivity of culturable deepsea-derived fungi from Okinawa Trough[J]. Journal of Oceanology and Limnology, 2021, 39(3): 892-902

Phylogenetic diversity and bioactivity of culturable deepsea-derived fungi from Okinawa Trough

Xiaoyong ZHANG1,2, Yiyang LI1, Zonghe YU1, Xiao LIANG2, Shuhua QI2,3
1 Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China;
2 Key Laboratory of Tropical Marine Bio-resources and Ecology/RNAM Center for Marine Microbiology/Guangdong Key Laboratory of Marine Material Medical, South China sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
3 Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou), Guangzhou 511458, China
Deep-sea sediments are now recognized as a home for rich and largely microbial community. Recently, it has been believed in an increasing number of studies that bacteria could be abundant in deepsea sediments of many types; however, fungi in deep-sea sediments remain relatively unknown. The phylogenetic diversity and bioactivity of culturable deep-sea-derived fungi from Okinawa Trough sediments were investigated in traditional method combined with fungal identification of molecular biology in this study. A total of 76 isolates belonged to 15 fungal taxa were recovered in a harsh condition of low nutrient and low temperature, indicating that the fungal communities in deep-sea sediments from Okinawa Trough were relatively abundant and diversified. Aspergillus, Cladosporium, and Penicillium were the dominant fungal genera, while Mycosphaerella, Purpureocillium, and Schizophyllum were relatively rare in the deep-sea sediments from Okinawa Trough. Among the six genera recovered, Mycosphaerella was firstly recovered from deep-sea sediments in this study. Moreover, about 75% of the extracts from the 15 fungal representative isolates displayed distinct bioactivity against at least one indicator bacterium or marine macrofouler, emphasizing the potentials of these deep-sea-derived fungi from Okinawa Trough as producers of bioactive metabolites. Notably, isolates Cladosporium oxysporum SCSIO z001 and Penicillium citrinum SCSIO z049 displayed a wide spectrum of bioactivities, isolates Cladosporium cladosporioides SCSIO z015, Cladosporium sphaerospermum SCSIO z030, and Penicillium verruculosum SCSIO z007 exhibited a strong anti-bacterial-growth activity, and isolate Penicillium chrysogenum SCSIO z062 displayed a strong anti-larval-settlement activity. These results suggest that these isolates deserved further study as potential sources of novel bioactive metabolites.
Key words:    deep-sea-derived fungi|phylogenetic diversity|bioactivity|Okinawa Trough|hydrothermal vents   
Received: 2020-01-29   Revised: 2020-04-02
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