Protective efficacy evaluation of immunogenic protein AHA_3793 of <i>Aeromonas hydrophila</i> as vaccine candidate for largemouth bass <i>Micropterus salmoides</i> -- Journal of Oceanology and Limnology,2023,41(1):392-400

	Cite this paper:
	Shun YANG, Idefonce MKINGULE, Long LIU, Wenqi CHEN, Xiangyu YUAN, Zixuan MA, Liang LIANG, Shichao QIAN, Mengmeng HUANG, Hui FEI. Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides[J]. Journal of Oceanology and Limnology, 2023, 41(1): 392-400

Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

Shun YANG¹, Idefonce MKINGULE¹, Long LIU², Wenqi CHEN¹, Xiangyu YUAN¹, Zixuan MA¹, Liang LIANG¹, Shichao QIAN³, Mengmeng HUANG¹, Hui FEI¹

1 College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China;
2 Zhejiang Development&Planning Institute, Hangzhou 310012, China;
3 Huzhou Baijiayu Biotech Co., Ltd., Huzhou 313000, China

Abstract:

Aeromonas hydrophila is a Gram-negative pathogen that can infect various fish, including largemouth bass (Micropterus salmoides), which have caused huge economic losses. In present study, largemouth bass anti-A. hydrophila antibodies were produced, then a highly immunogenic outer membrane proteins, AHA_3793, was identified by combined western blotting and mass spectrometry analysis. Moreover, AHA_3793 was expressed, and its immunogenicity was further verified by western blotting. Subsequently, the protective efficacy of AHA_3793 were evaluated in largemouth bass. The results showed that rAHA_3793 could produce a relative percentage survival (RPS) of 61.76% for largemouth bass against A. hydrophila challenge. ELISA analysis showed the specific serum antibodies of largemouth bass against rAHA_3793 and A. hydrophila in vaccinated group in weeks 4 and 5 after immunization were significantly higher than those in control group, which suggested that rAHA_3793 induced production of specific serum antibodies against rAHA_3793 and A. hydrophila. The qRT-PCR analysis showed that expressions of CD4-2 and MHC IIα were also significantly up-regulated after immunization. These results collectively demonstrated that rAHA_3793 could induce a strong humoral immune response of largemouth bass, and then produce high immune protection effects against A. hydrophila infection.

Key words: Micropterus salmoides|Aeromonas hydrophila|AHA_3793|subunit vaccine|immune response

Received: 2021-10-12 Revised:

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Articles by Shun YANG

Articles by Idefonce MKINGULE

Articles by Long LIU

Articles by Wenqi CHEN

Articles by Xiangyu YUAN

Articles by Zixuan MA

Articles by Liang LIANG

Articles by Shichao QIAN

Articles by Mengmeng HUANG

Articles by Hui FEI

References:
Abdelhamed H, Banes M, Karsi A et al. 2019. Recombinant ATPase of virulent Aeromonas hydrophila protects channel catfish against motile Aeromonas septicemia.Frontiers in Immunology, 10: 1641, https://doi.org/10.3389/fimmu.2019.01641.
Abdelhamed H, Ibrahim I, Nho S W et al. 2017. Evaluation of three recombinant outer membrane proteins, OmpA1, Tdr, and TbpA, as potential vaccine antigens against virulent Aeromonas hydrophila infection in channel catfish(Ictalurus punctatus). Fish & Shellfish Immunology, 66:480-486, https://doi.org/10.1016/j.fsi.2017.05.043.
Blazer V S, Iwanowicz L R, Starliper C E et al. 2010. Mortality of centrarchid fishes in the Potomac drainage: survey results and overview of potential contributing factors.Journal of Aquatic Animal Health, 22(3): 190-218, https://doi.org/10.1577/H10-002.1.
Chida A S, Goyos A, Robert J. 2011. Phylogenetic and developmental study of CD4, CD8 α and β T cell coreceptor homologs in two amphibian species, Xenopus tropicalis and Xenopus laevis. Developmental & Comparative Immunology, 35(3): 366-377, https://doi.org/10.1016/j.dci.2010.11.005.
Dubin A, Jørgensen T E, Moum T et al. 2019. Complete loss of the MHC II pathway in an anglerfish, Lophius piscatorius. Biology Letters, 15(10): 20190594, https://doi.org/10.1098/rsbl.2019.0594.
Feng J J, Lin P, Guo S L et al. 2017. Identification and characterization of a novel conserved 46 kD maltoporin of Aeromonas hydrophila as a versatile vaccine candidate in European eel (Anguilla anguilla). Fish & Shellfish Immunology, 64: 93-103, https://doi.org/10.1016/j.fsi.2017.03.010.
Grimholt U. 2016. MHC and evolution in teleosts. Biology, 5(1): 6, https://doi.org/10.3390/biology5010006.
Guan Q F, Bhowmick B, Upadhyay A et al. 2021. Structure and functions of bacterial outer membrane protein A, a potential therapeutic target for bacterial infection. Current Topics in Medicinal Chemistry, 21(13): 1129-1138, https://doi.org/10.2174/1568026621666210705164319.
Guan R Z, Xiong J, Huang W S et al. 2011. Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein. Acta Biochimica et Biophysica Sinica, 43(1): 79-88, https://doi.org/10.1093/abbs/gmq115.
Guo Z, Lin Y X, Wang X Y et al. 2018. The protective efficacy of four iron-related recombinant proteins and their single-walled carbon nanotube encapsulated counterparts against Aeromonas hydrophila infection in zebrafish.Fish & Shellfish Immunology, 82: 50-59, https://doi.org/10.1016/j.fsi.2018.08.009.
Han B Q, Xu K, Liu Z T et al. 2019. Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus. Fish & Shellfish Immunology, 84: 948-954, https://doi.org/10.1016/j.fsi.2018.10.065.
Kalita P, Lyngdoh D L, Padhi A K et al. 2019. Development of multi-epitope driven subunit vaccine against Fasciola gigantica using immunoinformatics approach. International Journal of Biological Macromolecules, 138:224-233, https://doi.org/10.1016/j.ijbiomac.2019.07.024.
Li M, Zhou H, Yang C et al. 2020. Bacterial outer membrane vesicles as a platform for biomedical applications: an update. Journal of Controlled Release, 323: 253-268, https://doi.org/10.1016/j.jconrel.2020.04.031.
Liu F G, Tang X Q, Sheng X Z et al. 2016. Edwardsiella tarda outer membrane protein C: an immunogenic protein induces highly protective effects in flounder (Paralichthys olivaceus) against Edwardsiellosis. International Journal of Molecular Sciences, 17(7): 1117, https://doi.org/10.3390/ijms17071117.
Lopez P, Guaimas F, Czibener C et al. 2020. A genomic island in Brucella involved in the adhesion to host cells:identification of a new adhesin and a translocation factor.Cellular Microbiology, 22(11): e13245, https://doi.org/10.1111/cmi.13245.
Parker J L, Shaw J G. 2011. Aeromonas spp. clinical microbiology and disease. Journal of Infection, 62(2):109-118, https://doi.org/10.1016/j.jinf.2010.12.003.
Passalia F J, Carvalho E, Heinemann M B et al. 2020. The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components. Microbiological Research, 235: 126470, https://doi.org/10.1016/j.micres.2020.126470.
Sheng X Z, Liu M, Liu H B et al. 2018. Identification of immunogenic proteins and evaluation of recombinant PDHA1 and GAPDH as potential vaccine candidates against Streptococcus iniae infection in flounder(Paralichthys olivaceus). PLoS One, 13(5): e0195450, https://doi.org/10.1371/journal.pone.0195450.
Wang C, Hu Y H, Chi H et al. 2013a. The major fimbrial subunit protein of Edwardsiella tarda: vaccine potential, adjuvant effect, and involvement in host infection. Fish & Shellfish Immunology, 35(3): 858-865, https://doi.org/10.1016/j.fsi.2013.06.021.
Wang N, Yang Z, Zang M F et al. 2013b. Identification of Omp38 by immunoproteomic analysis and evaluation as a potential vaccine antigen against Aeromonas hydrophila in Chinese breams. Fish & Shellfish Immunology, 34(1): 74-81, https://doi.org/10.1016/j.fsi.2012.10.003.
Wang Y Q, Chen H R, Guo Z et al. 2017. Quantitative proteomic analysis of iron-regulated outer membrane proteins in Aeromonas hydrophila as potential vaccine candidates. Fish & Shellfish Immunology, 68: 1-9, https://doi.org/10.1016/j.fsi.2017.07.002.
Xing J, Xu H S, Wang Y et al. 2017a. Identification of immunogenic proteins and evaluation of four recombinant proteins as potential vaccine antigens from Vibrio anguillarum in flounder (Paralichthys olivaceus).Vaccine, 35(24): 3196-3203, https://doi.org/10.1016/j.vaccine.2017.04.071.
Xing J, Xu H S, Wang Y et al. 2017b. Protective efficacy of six immunogenic recombinant proteins of Vibrio anguillarum and evaluation them as vaccine candidate for flounder (Paralichthys olivaceus). Microbial Pathogenesis, 107: 155-163, https://doi.org/10.1016/j.micpath.2017.03.027.
Xu T J, Chen S L, Zhang Y X. 2010. MHC class IIα gene polymorphism and its association with resistance/susceptibility to Vibrio anguillarum in Japanese flounder (Paralichthys olivaceus). Developmental & Comparative Immunology, 34(10): 1042-1050, https://doi.org/10.1016/j.dci.2010.05.008.
Yadav S K, Dash P, Sahoo P K et al. 2018. Modulation of immune response and protective efficacy of recombinant outer-membrane protein F (rOmpF) of Aeromonas hydrophila in Labeo rohita. Fish & Shellfish Immunology, 80: 563-572, https://doi.org/10.1016/j.fsi.2018.06.041.
Yang S, Chen W Q, He F F et al. 2020. Comparison of the roles of IgM in systemic and mucosal immunity via tissue distribution analysis in largemouth bass (Micropterus salmoides). Aquaculture, 527: 735488, https://doi.org/10.1016/j.aquaculture.2020.735488.
Yuan X Y, Zhang X T, Xia Y T et al. 2021. Transcriptome and 16S rRNA analyses revealed differences in the responses of largemouth bass (Micropterus salmoides) to early Aeromonas hydrophila infection and immunization.Aquaculture, 541: 736759, https://doi.org/10.1016/j.aquaculture.2021.736759.
Yun S, Jun J W, Giri S S et al. 2017. Efficacy of PLGA microparticle-encapsulated formalin-killed Aeromonas hydrophila cells as a single-shot vaccine against A. hydrophila infection. Vaccine, 35(32): 3959-3965, https://doi.org/10.1016/j.vaccine.2017.06.005.
Zhang D H, Xu D H, Beck B. 2019. Analysis of agglutinants elicited by antiserum of channel catfish immunized with extracellular proteins of virulent Aeromonas hydrophila.Fish & Shellfish Immunology, 86: 223-229, https://doi.org/10.1016/j.fsi.2018.11.033.
Zhang H W, Qian P, Peng B et al. 2015. A novel subunit vaccine co-expressing GM-CSF and PCV2b Cap protein enhances protective immunity against porcine circovirus type 2 in piglets. Vaccine, 33(21): 2449-2456, https://doi.org/10.1016/j.vaccine.2015.03.090.
Zhao L N, Tang X Q, Sheng X Z et al. 2020. Different immune responses of flounder (Paralichthys olivaceus) towards the full-length and N-terminal or C-terminal portion of hirame novirhabdovirus glycoprotein. Fish & Shellfish Immunology, 104: 279-288, https://doi.org/10.1016/j.fsi.2020.06.002.
Zhong Y F, Shi C M, Zhou Y L et al. 2020. Optimum dietary fiber level could improve growth, plasma biochemical indexes and liver function of largemouth bass, Micropterus salmoides. Aquaculture, 518: 734661, https://doi.org/10.1016/j.aquaculture.2019.734661.
Zhou J, Zhao H, Zhang L et al. 2021. MiRNA-seq analysis of spleen and head kidney tissue from aquacultured largemouth bass (Micropterus salmoides) in response to Aeromonas hydrophila infection. Functional & Integrative Genomics, 21(1): 101-111, https://doi.org/10.1007/s10142-020-00763-8.