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Cite this paper: |
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Songze KE, Bo ZHANG, Yanlei YU, Sijia WANG, Weihua JIN, Jian WU, Jianwei CHEN, Huawei ZHANG, Bin WEI, Hong WANG. Structural characterization of sulfated galactofucan from Undaria pinnatifida and its effect on type 2 diabetic mice[J]. Journal of Oceanology and Limnology, 2023, 41(1): 300-313 |
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Structural characterization of sulfated galactofucan from Undaria pinnatifida and its effect on type 2 diabetic mice |
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| Songze KE1, Bo ZHANG1, Yanlei YU1,4, Sijia WANG1,2, Weihua JIN3, Jian WU5, Jianwei CHEN1,4, Huawei ZHANG1,4, Bin WEI1,4, Hong WANG1,4 |
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1 College of Pharmaceutical Science&Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Moganshan Research Institute at Deqing County Zhejiang University of Technology, Zhejiang University of Technology, Hangzhou 310014, China;
2 Center for Human Nutrition, David Geffen School of Medicine, University of California, Los Angeles CA 90024, USA;
3 College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China;
4 Key Laboratory of Marine Fishery Resources Exploitment&Utilization of Zhejiang Province, Hangzhou 310014, China;
5 Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology, Hangzhou 310014, China |
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| Abstract: |
| A sulfated galactofucan designated as UpG was obtained from the brown algae Undaria pinnatifida by calcium chloride extraction. Chemical analyses showed that UpG is composed of galactose and fucose at a high sulfation level. Low-molecular weight UpGP-0.5 was prepared from UpG through acid hydrolysis for structure characterization. The backbones of UpG are determined to be α-(1,3)-Fuc, α-(1,4)-Gal, α-(1,3)-Gal, and α-(1,6)-Gal by GC-MS, FT-IR, NMR, and LC-MS analyses. Sulfate groups are modified at C2 and/or C4 of fucose and C3 and/or C4 of galactose. UpG could partially lower blood sugar and serum lipid levels in type 2 diabetic mice. Moreover, UpG treatment regulates the abundance of some specific gut microbiota, such as enriching the abundance of Muribaculum and Christensenellaceae, and reducing that of Bilophila, Tannerellaceae, Candidatus Saccharimonas and Anaerotruncus. The findings characterized the detailed structure of a sulfated galactofucan and investigated its potential for the treatment of type 2 diabetes mellitus. |
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| Key words:
Undaria pinnatifida|galactofucan|structure|gut microbiota|diabetes
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| Received: 2021-09-19 Revised: |
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