Variations in nano- and pico-eukaryotic phytoplankton assemblages in the Qinhuangdao green-tide area -- Journal of Oceanology and Limnology,2022,40(6):2446-2461

	Cite this paper:
	Weiqian ZHANG, Hongbin HAN, Limei QIU, Chao LIU, Qingchun ZHANG, Guizhong ZHOU. Variations in nano- and pico-eukaryotic phytoplankton assemblages in the Qinhuangdao green-tide area[J]. Journal of Oceanology and Limnology, 2022, 40(6): 2446-2461

Variations in nano- and pico-eukaryotic phytoplankton assemblages in the Qinhuangdao green-tide area

Weiqian ZHANG^1,2,3, Hongbin HAN⁶, Limei QIU^2,3,8, Chao LIU^4,5,7, Qingchun ZHANG^4,5,8, Guizhong ZHOU¹

1 Qingdao University of Science and Technology, Qingdao 266071, China;
2 CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, CAS Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
3 Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;
4 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
5 Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;
6 Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China;
7 University of Chinese Academy of Sciences, Beijing 100049, China;
8 Center of Ocean Mega Science, Chinese Academy of Sciences, Qingdao 266071, China

Abstract:

Qinhuangdao coastal waters have been frequently hitting by nano- and pico-eukaryotic phytoplankton (NPEP) blooms and green tides (macroalgal blooms) in the recent decade. However, understanding about the impacts of environmental factors and the green tides on the NPEP assemblages in this area is limited. In this study, the composition of NPEP assemblages and their variations were analyzed via amplicon sequence variants (ASVs) assay based on amplicon high-throughput sequencing data with the 18S V4 region as a targeted gene in the Qinhuangdao green-tide area during the green tide. Consequently, average NPEP effective sequences and ASVs of 178 000 and 200 were obtained from each sample, respectively. Although there were 25 classes, 110 genera, and 97 species of NPEP were identified and annotated, the proportions of annotated ASVs at genus and species levels were only 44.7% and 17.8%, respectively. The NPEP communities had a seasonal succession from diatom-dominated to dinoflagellate-dominated. During the three investigations, Skeletonema, Karlodinium, and Gonyaulax were the most dominant genera in May, August, and September, respectively. Species diversity and the abundance of NPEP communities could be increased by a high content of dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP) but inhibited by low dissolved inorganic phosphorus content. The outbreak of green tides could alter the composition and content of nutrients and accelerate the succession of the NPEP communities from diatom-dominated to dinoflagellate-dominated under the background of a seasonal increase in seawater temperature. These results preliminarily revealed the impacts of the recurrent occurrences of green tides on the NPEP assemblages in the Qinhuangdao green-tide area exhibiting high DON content and dissolved inorganic nitrogen/phosphorus ratio.

Key words: nano- and pico-eukaryotic phytoplankton|high-throughput sequencing|green tide|eutrophication|Qinhuangdao

Received: 2022-04-19 Revised:

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Articles by Hongbin HAN

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Articles by Qingchun ZHANG

Articles by Guizhong ZHOU

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