|
|
Cite this paper: |
|
|
Xianzhe WANG, Xingye HAN, Hongmei GE. Effect of light intensity on bound EPS characteristics of two Microcystis morphospecies: the role of bEPS in the proliferation of Microcystis[J]. Journal of Oceanology and Limnology, 2022, 40(5): 1706-1719 |
|
|
|
|
|
|
|
Effect of light intensity on bound EPS characteristics of two Microcystis morphospecies: the role of bEPS in the proliferation of Microcystis |
|
| Xianzhe WANG1,2, Xingye HAN1,2, Hongmei GE1,2 |
|
1 School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068, China;
2 Hubei Key Laboratory of Ecological Restoration for River-Lakes and Algal Utilization, Wuhan 430068, China |
|
| Abstract: |
| Bound extracellular polymeric substances (bEPS) play an important role in the proliferation of Microcystis. However, the understanding of bEPS characterization remains limited. In this study, threedimensional fluorescence excitation-emission matrix (3D-EEM) spectroscopy and zeta potentiometer were used to characterize the loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) from two dominant Microcystis morphospecies from Taihu Lake (China) at different light intensities. Physiochemical analysis showed that the growth and TB-EPS or bEPS contents in Microcystis aeruginosa were higher than those in Microcystis flos-aquae at each light intensity. The 3D-EEM contour demonstrated that the intensities of peak B (tryptophan-like substances) in the TB-EPS from M. aeruginosa were stronger than those from M. flosaquae when the light intensity was higher than 10 μE/(m2·s). Zeta potential analysis showed that the absolute values of the zeta potential of TB-EPS in the two species both increased with rising light intensity, except those of TB-EPS in M. aeruginosa at 105 μE/(m2·s). Moreover, the absolute values of the zeta potential of M. aeruginosa were higher than those of M. flos-aquae at each light intensity. All these results indicated that M. aeruginosa may more quickly proliferate than M. flos-aquae through increased negative charges, bEPS contents, growth, and tryptophan-like substance contents at certain light intensities. |
|
| Key words:
Microcystis
bound extracellular polymeric substances
light intensity
zeta potential
|
|
| Received: 2021-10-30 Revised: 2022-01-04 |
|
|
|
|
References:
|
|
|
|