Description of two new species of <i>Pseudoaliinostoc</i> (Nostocales, Cyanobacteria) from China based on the polyphasic approach -- Journal of Oceanology and Limnology,2022,40(3):1233-1244

	Cite this paper:
	Fangfang CAI, Gongliang YU, Renhui LI. Description of two new species of Pseudoaliinostoc (Nostocales, Cyanobacteria) from China based on the polyphasic approach[J]. Journal of Oceanology and Limnology, 2022, 40(3): 1233-1244

Description of two new species of Pseudoaliinostoc (Nostocales, Cyanobacteria) from China based on the polyphasic approach

Fangfang CAI^1,2, Gongliang YU², Renhui LI³

1 Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China;
2 Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
3 School of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China

Abstract:

Two cyanobacterial strains CHAB5870 and CHAB5871 morphologically identified as Nostoclike species were isolated from different habitats in China, and they were phylogenetically and taxonomically characterized based on a polyphasic approach combining morphological, ecological, and molecular data. In the 16S rRNA gene phylogeny inferred using maximum likelihood, maximum-parismony, and bayesian inference methods, these two strains clustered within the Pseudoaliinostoc clade. The 16S rRNA gene sequences of these two strains displayed ≥95.5% and ≤98% similarity to Pseudoaliinostoc species, which indicated them to represent new species of the genus Pseudoaliinostoc. Furthermore, the unique pattern of D1-D1' and Box-B helix of the 16S-23S rRNA internal transcribed spacer (ITS) secondary structure also revealed that two strains represented novel species. These results supported the establishment of two new Pseudoaliinostoc species with the name P. jiangxiense sp. nov. and P. yunnanense sp. nov.

Received: 2021-03-31 Revised:

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Articles by Gongliang YU

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References:
Bagchi S N, Dubey N, Singh P.2017.Phylogenetically distant clade of Nostoc-like taxa with the description of Aliinostoc gen.nov.and Aliinostoc morphoplasticum sp.nov.International Journal of Systematic and Evolutionary Microbiology, 67(9):3329-3338, https://doi.org/10.1099/ijsem.0.002112.
Bohunická M, Pietrasiak N, Johansen J R, Gómez E B, Hauer T, Gaysina L A, Lukešová A.2015.Roholtiella, gen.nov.(Nostocales, Cyanobacteria)-a tapering and branching cyanobacteria of the family Nostocaceae.Phytotaxa, 197(2):84-103, https://doi.org/10.11646/phytotaxa.197.2.2.
Cai F F, Li R H.2020.Purpureonostoc, a new name for a recently described genus of Nostoc-like cyanobacteria.Fottea, 20(2):111, https://doi.org/10.5507/fot.2020.007.
Cai F F, Li X C, Geng R Z, Peng X, Li R H.2019a.Phylogenetically distant clade of Nostoc-like taxa with the description of Minunostoc gen.nov.and Minunostoc cylindricum sp.nov.Fottea, 19(1):13-24, https://doi.org/10.5507/fot.2018.013.
Cai F F, Li X C, Yang Y M, Jia N N, Huo D, Li R H.2019b.
Compactonostoc shennongjiaensis gen.& sp.nov.(Nostocales, Cyanobacteria) from a wet rocky wall in China.Phycologia, 58(2):200-210, https://doi.org/10.108 0/00318884.2018.1541270.
Cai F F, Peng X, Li R H.2020a.Violetonostoc minutum gen.et sp.nov.(Nostocales, Cyanobacteria) from a rocky substrate in China.Algae, 35(1):1-15, https://doi.org/10.4490/algae.2020.35.3.4.
Cai F F, Wang Y L, Yu G L, Wang J, Peng X, Li R H.2020b.Proposal of Purpurea gen.nov.(Nostocales, cyanobacteria), a novel cyanobacterial genus from wet soil samples in Tibet, China.Fottea, 20(1):86-97, https://doi.org/10.5507/fot.2019.018.
Choi H J, Joo J H, Kim J H, Wang P B, Ki J S, Han M S.2018.Morphological characterization and molecular phylogenetic analysis of Dolichospermum hangangense(Nostocales, Cyanobacteria) sp.nov.from Han River, Korea.Algae, 33(2):143-156, https://doi.org/10.4490/algae.2018.33.5.2.
Clarke J D.2009.Cetyltrimethyl ammonium bromide (CTAB)DNA miniprep for plant DNA isolation.Cold Spring Harbor Protocols, 2009(3):pdb.prot5177, https://doi.org/10.1101/pdb.prot5177.
Edwards U, Rogall T, Blöcker H, Emde M, Böttger E C.1989.Isolation and direct complete nucleotide determination of entire genes.Characterization of a gene coding for 16S ribosomal RNA.Nucleic Acids Research, 17(19):7843-7853, https://doi.org/10.1093/nar/17.19.7843.
Genuário D B, Corrêa D M, Komárek J, Fiore M F.2013.Characterization of freshwater benthic biofilm-forming Hydrocoryne (Cyanobacteria) isolates from Antarctica.Journal of Phycology, 49(6):1142-1153, https://doi.org/10.1111/jpy.12124.
Genuário D B, Vaz M G M V, Hentschke G S, Sant'Anna C L, Fiore M F.2015.Halotia gen.nov., a phylogenetically and physiologically coherent cyanobacterial genus isolated from marine coastal environments.International Journal of Systematic and Evolutionary Microbiology, 65(Pt_2):663-675, https://doi.org/10.1099/ijs.0.070078-0.
Gkelis S, Rajaniemi P, Vardaka E, Moustaka-Gouni M, Lanaras T, Sivonen K.2005.Limnothrix redekei (Van Goor)Meffert (Cyanobacteria) strains from Lake Kastoria, Greece form a separate phylogenetic group.Microbial Ecology, 49(1):176-182, https://doi.org/10.1007/s00248-003-2030-7.
Hentschke G S, Johansen J R, Pietrasiak N, Rigonato J, Fiore M F, Sant'anna C L.2017.Komarekiella atlantica gen.et sp.nov.(Nostocaceae, Cyanobacteria):a new subaerial taxon from the Atlantic Rainforest and Kauai, Hawaii.Fottea, 17(2):178-190, https://doi.org/10.5507/fot.2017.002.
Hrouzek P, Lukešová A, Mareš J, Ventura S.2013.Description of the cyanobacterial genus Desmonostoc gen.nov.including D.muscorum comb.nov.as a distinct, phylogenetically coherent taxon related to the genus Nostoc.Fottea, 13(2):201-213, https://doi.org/10.5507/fot.2013.016.
Hrouzek P, Ventura S, Lukešová A, Mugnai M, Angela Turicchia S, Komárek J.2005.Diversity of soil Nostoc strains:phylogenetic and phenotypic variability.Algological Studies, (117):251-264, https://doi.org/10.1127/1864-1318/2005/0117-0251.
Iteman I, Rippka R, De Marsac N T, Herdman M.2000.Comparison of conserved structural and regulatory domains within divergent 16S rRNA-23S rRNA spacer sequences of cyanobacteria.Microbiology, 146(6):1275-1286, https://doi.org/10.1099/00221287-146-6-1275.
Johansen J R, Casamatta D A.2005.Recognizing cyanobacterial diversity through adoption of a new species paradigm.Algological Studies, (117):71-93, https://doi.org/10.1127/1864-1318/2005/0117-0071.
Johansen J R, Kovacik L, Casamatta D A, Iková K F, Kaštovský J.2011.Utility of 16S-23S ITS sequence and secondary structure for recognition of intrageneric and intergeneric limits within cyanobacterial taxa:Leptolyngbya corticola sp.nov.(Pseudanabaenaceae, Cyanobacteria).Nova Hedwigia,92(3-4):283-302, https://doi.org/10.1127/0029-5035/2011/0092-0283.
Kabirnataj S, Nematzadeh G A, Talebi A F, Saraf A, Suradkar A, Tabatabaei M, Singh P.2020.Description of novel species of Aliinostoc, Desikacharya and Desmonostoc using a polyphasic approach.International Journal of Systematic and Evolutionary Microbiology, 70(5):3413-3426, https://doi.org/10.1099/ijsem.0.004188.
Kalyaanamoorthy S, Minh B Q, Wong T K F, Von Haeseler A, Jermiin L S.2017.ModelFinder:fast model selection for accurate phylogenetic estimates.Nature Methods, 14(6):587-589, https://doi.org/10.1038/nmeth.4285.
Katoh K, Standley D M.2013.MAFFT multiple sequence alignment software version 7:improvements in performance and usability.Molecular Biology and Evolution, 30(4):772-780, https://doi.org/10.1093/molbev/mst010.
Kim M, Oh H S, Park S C, Chun J.2014.Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes.International Journal of Systematic and Evolutionary Microbiology, 64(Pt_2):346-351, https://doi.org/10.1099/ijs.0.059774-0.
Komárek J, Kaštovský J, Mareš J, Johansen J R.2014.Taxonomic classification of cyanoprokaryotes(cyanobacterial genera) 2014, using a polyphasic approach.Preslia, 86(4):295-335.
Komárek J.2003.Problem of the taxonomic category "species" in cyanobacteria.Algological Studies, (109):281-297, https://doi.org/10.1127/1864-1318/2003/0109-0281.
Komárek J.2016.A polyphasic approach for the taxonomy of cyanobacteria:principles and applications.European Journal of Phycology, 51(3):346-353, https://doi.org/10.1080/09670262.2016.1163738.
Komárek J.2018.Several problems of the polyphasic approach in the modern cyanobacterial system.Hydrobiologia, 811(1):7-17, https://doi.org/10.1007/s10750-017-3379-9.
Komárek J.2020.Quo vadis, taxonomy of cyanobacteria(2019).Fottea, 20(1):104-110, https://doi.org/10.5507/fot.2019.020.
Kozhevnikov I V, Kozhevnikova N A.2011.Phylogenetic and morphological evaluation of Wollea saccata (Nostocales, Cyanobacteria) isolated from the Yenissei River basin(Eastern Siberia, Russia).Fottea, 11(1):99-106, https://doi.org/10.5507/fot.2011.010.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K.2018.MEGA X:molecular evolutionary genetics analysis across computing platforms.Molecular Biology and Evolution, 35(6):1547-1549, https://doi.org/10.1093/molbev/msy096.
Kumar S, Stecher G, Tamura K.2016.MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets.Molecular Biology and Evolution, 33(7):1870-1874, https://doi.org/10.1093/molbev/msw054.
Lee N J, Bang S D, Kim T, Ki J S, Lee O M.2021.Pseudoaliinostoc sejongens gen.& sp.nov.(Nostocales, Cyanobacteria) from floodplain soil of the Geum River in Korea based on polyphasic approach.Phytotaxa, 479(1):55-70, https://doi.org/10.11646/phytotaxa.479.1.4.
Lukešová A, Johansen J R, Martin M P, Casamatta D A.2009.Aulosira bohemensis sp.nov.:further phylogenetic uncertainty at the base of the Nostocales (Cyanobacteria).Phycologia, 48(2):118-129, https://doi.org/10.2216/08-56.1.
Mai T, Johansen J R, Pietrasiak N, Bohunická M, Martin M P.2018.Revision of the Synechococcales (cyanobacteria) through recognition of four families including Oculatellaceae fam.nov.and Trichocoleaceae fam.nov.and six new genera containing 14 species.Phytotaxa, 365(1):1-59, https://doi.org/10.11646/phytotaxa.365.1.1.Mathews Lab.2021.RNAstructure, version 6.3.http://rna.urmc.rochester.edu/RNAstructure.html.
Miller M A, Schwartz T, Pickett B, He S, Klem E B, Scheuermann R H, Passarotti M, Kaufman S, O'Leary M A.2015.A RESTful API for access to phylogenetic tools via the CIPRES science gateway.Evolutionary Bioinformatics, 11:43-48, https://doi.org/10.4137/EBO.S21501.
Miscoe L H, Johansen J R, Vaccarino M A, Pietrasiak N, Sherwood A R.2016.Novel cyanobacteria from caves on Kauai, Hawaii.In:Miscoe L H ed.The Diatom Flora and Cyanobacteria from Caves on Kauai, Hawaii:Taxonomy, Distribution, New Species.Bibliotheca Phycologica, Stuttgart.p.75-152.
Osorio-Santos K, Pietrasiak N, Bohunická M, Miscoe L H, Kováčik L, Martin M P, Johansen J R.2014.Seven new species of Oculatella (Pseudanabaenales, Cyanobacteria):taxonomically recognizing cryptic diversification.European Journal of Phycology, 49(4):450-470, https://doi.org/10.1080/09670262.2014.976843.
Papaefthimiou D, Hrouzek P, Mugnai M A, Lukesova A, Turicchia S, Rasmussen U, Ventura S.2008.Differential patterns of evolution and distribution of the symbiotic behaviour in nostocacean cyanobacteria.International Journal of Systematic and Evolutionary Microbiology, 58(3):553-564, https://doi.org/10.1099/ijs.0.65312-0.
Rajaniemi P, Hrouzek P, Kaštovská K, Willame R, Rantala A, Hoffmann L, Komárek J, Sivonen K.2005.Phylogenetic and morphological evaluation of the genera Anabaena, Aphanizomenon, Trichormus and Nostoc (Nostocales, Cyanobacteria).International Journal of Systematic and Evolutionary Microbiology, 55(1):11-26, https://doi.org/10.1099/ijs.0.63276-0.
Rambaut A.2016.Tree figure drawing tool Version 1.4.3.Institute of Evolutionary Biology, United Kingdom, University of Edinburgh.https://vcru.wisc.edu/simonlab/bioinformatics/programs/fgtree/README.txt.
Řeháková K, Johansen J R, Casamatta D A, Xuesong L, Vincent J.2007.Morphological and molecular characterization of selected desert soil cyanobacteria:three species new to science including Mojavia pulchra gen.et sp.nov.Phycologia, 46:481-502.
Ronquist F, Teslenko M, Van Der Mark P, Ayres D L, Darling A, Höhna S, Larget B, Liu L, Suchard M A, Huelsenbeck J P.2012.MrBayes 3.2:Efficient bayesian phylogenetic inference and model choice across a large model space.Systematic Biology, 61(3):539-542, https://doi.org/10.1093/sysbio/sys029.
Sambrook J, Russell D W.2001.Molecular cloning:a laboratory manual.3rd ed.Cold Spring Harbor Laboratory Press, New York.p.1.84-1.87.
Saraf A G, Dawda H G, Singh P.2019.Validation of the genus Desikacharya gen.nov.(Nostocaceae, Cyanobacteria)and three included species.Notulae Algarum, (107):1-3, figs 1-8, https://doi.org/10.1099/ijsem.0.001800.
Saraf A, Dawda H G, Suradkar A, Behere I, Kotulkar M, Shaikh Z M, Kumat A, Batule P, Mishra D, Singh P.2018.Description of two new species of Aliinostoc and one new species of Desmonostoc from India based on the polyphasic approach and reclassification of Nostoc punensis to Desmonostoc punense comb.nov.FEMS Microbiology Letters, 365(24):fny272, https://doi.org/10.1093/femsle/fny272.
Sciuto K, Moro I.2016.Detection of the new cosmopolitan genus Thermoleptolyngbya (Cyanobacteria, Leptolyngbyaceae) using the 16S rRNA gene and 16S-23S ITS region.Molecular Phylogenetics and Evolution, 105:15-35, https://doi.org/10.1016/j.ympev.2016.08.010.
Silva C S P, Genuário D B, Vaz M G M V, Fiore M F.2014.Phylogeny of culturable cyanobacteria from Brazilian mangroves.Systematic and Applied Microbiology, 37(2):100-112, https://doi.org/10.1016/j.syapm.2013.12.003.
Stackebrandt E, Ebers J.2006.Taxonomic parameters revisited:tarnished gold standards.Microbiology Today, 33:152-155.
Stackebrandt E, Goebel B M.1994.Taxonomic note:a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology.International Journal of Systematic and Evolutionary Microbiology, 44(4):846-849, https://doi.org/10.1099/00207713-44-4-846.
Taton A, Grubisic S, Brambilla E, De Wit R, Wilmotte A.2003.Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica):a morphological and molecular approach.Applied and Environmental Microbiology, 69(9):5157-5169, https://doi.org/10.1128/AEM.69.9.5157-5169.2003.
Taton A, Grubisic S, Ertz D, Hodgson D A, Piccardi R, Biondi N, Tredici M R, Mainini M, Losi D, Marinelli F, Wilmotte A.2006.Polyphasic study of Antarctic cyanobacterial strains.Journal of Phycology, 42(6):1257-1270, https://doi.org/10.1111/j.1529-8817.2006.00278.x.
Trifinopoulos J, Nguyen L T, Von Haeseler A, Minh B Q.2016.W-IQ-TREE:a fast online phylogenetic tool for maximum likelihood analysis.Nucleic Acids Research, 44(W1):W232-W235, https://doi.org/10.1093/nar/gkw256.
Turicchia S, Ventura S, Komárková J, Komárek J.2009.Taxonomic evaluation of cyanobacterial microflora from alkaline marshes of northern Belize.2.Diversity of oscillatorialean genera.Nova Hedwigia, 89(1-2):165-200, https://doi.org/10.1127/0029-5035/2009/0089-0165.
Vaccarino M A, Johansen J R.2012.Brasilonema angustatum sp.nov.(Nostocales), a new filamentous cyanobacterial species from the Hawaiian Islands.Journal of Phycology, 48(5):1178-1186, https://doi.org/10.1111/j.1529-8817.2012.01203.x.
Watanabe M M, Ichimura T.1977.Fresh-and salt-water forms of Spirulina platensis in axenic cultures.Bulletin of Japanese Society of Phycology, 25:371-377.
Zapomělová E, Mareš J, Lukešová A, Skácelová O, Řeháková K, Kust A.2013.Extensive polyphyly of non-gasvacuolate Anabaena species (Nostocales, Cyanobacteria):a challenge for modern cyanobacterial taxonomy.In:Book of abstracts, 19th Symposium of the International Society for Cyanophyte Research, p.51, Cleveland, Ohio.